brazilian wandering spider cost

Brazilian wandering spiders: Bites & other facts

The spider's name means "murderess" in Greek, which is appropriate for the deadly arachnid.

Classification/taxonomy

Size & characteristics, bites and venom, additional resources.

The Brazilian wandering spider, also called armed spiders or banana spiders, belongs to the genus Phoneutria , which means "murderess" in Greek. And it's no wonder why — it's one of the most venomous spiders on Earth . Its bite, which delivers neurotoxic venom, can be deadly to humans, especially children, although antivenom makes death unlikely.

Guinness World Records has previously named the Brazilian wandering spider the world's most venomous spider multiple times (though the current record-holder is the Sydney funnel-web spider, Atrax robustus , according to Guinness ). But, as the late Jo-Anne Sewlal, who was an arachnologist at the University of the West Indies in Trinidad and Tobago, told Live Science, "classifying an animal as deadly is controversial," as the amount of damage depends on the amount of venom injected. 

Jo-Anne Sewlal was a noted arachnologist from Trinidad and Tobago. While completing her PhD, she received the National Institute of Higher Education, Research, Science and Technology (NIHERST) 2012 Award for Excellence in Science and Technology for Junior Scientist. In 2013, She received a doctorate in zoology from the University of the West Indies. She discovered several species of spiders in her home country, surveyed the arachnids across several countries the Caribbean and appeared as an expert on the topic on The Science Channel. She died of an allergic reaction in January 2020.

There are nine species of Brazilian wandering spider, all of which are nocturnal and can be found in Brazil. Some species also can be found throughout Central and South America, from Costa Rica to Argentina, according to a 2008 article in the journal American Entomologist . Study author Richard S. Vetter, a research associate in the department of entomology at the University of California, Agriculture and Natural Resources, wrote that specimens of these powerful arachnids have been mistakenly exported to North America and Europe in banana shipments. However, Vetter noted, in many cases of cargo infestation, the spider in question is a harmless banana spider (genus Cupiennius ) that is misidentified as a Phoneutria . The two types of spiders look similar.

The taxonomy of Brazilian wandering spiders, according to the Integrated Taxonomic Information System (ITIS) , is:

Kingdom : Animalia Subkingdom : Bilateria Infrakingdom : Protostomia Superphylum : Ecdysozoa Phylum : Arthropoda Subphylum : Chelicerata Class : Arachnida Order : Araneae Family : Ctenidae Genus : Phoneutria  

• Phoneutria bahiensis
• Phoneutria boliviensis
• Phoneutria eickstedtae
• Phoneutria fera
• Phoneutria keyserlingi
• Phoneutria nigriventer
• Phoneutria pertyi
• Phoneutria reidyi
• Phoneutria depilata , according to a 2021 study published in the journal ZooKeys , which found that Phoneutria boliviensis actually included two separate species from different habitats. 

Brazilian wandering spiders are large, with bodies reaching up to 2 inches (5 centimeters) and a leg span of up to 7 inches (18 cm), according to the Natural History Museum in Karlsruhe, Germany. The species vary in color, though all are hairy and mostly brown and gray, although some species have lightly colored spots on their abdomen. Many species have bands of black and yellow or white on the underside of the two front legs, according to the University of Florida . 

These arachnids "are called wandering spiders because they do not build webs but wander on the forest floor at night, actively hunting prey," Sewlal told Live Science in an interview conducted in 2014, before her death. They kill by both ambush and direct attack.

They spend most of their day hiding under logs or in crevices, and come out to hunt at night. They eat insects, other spiders and sometimes, small amphibians, reptiles and mice. 

Research into one species of Brazilian wandering spider, Phoneutria boliviensis , revealed that these spiders eat a mix of arthropods and reptiles. DNA metabarcoding, a technique that examines the DNA and RNA in a sample, of the guts of 57 spiders identified 96 prey species, including flies, beetles, butterflies, moths, grasshoppers, locusts and crickets, according to research from the University of Tolima and the University of Ibagué in Colombia . Some of the female spiders also ate lizards and snakes.

While their bites are powerful and painful, "their bites are a means of self-defense and only done if they are provoked intentionally or by accident," Sewlal said.

In the Brazilian wandering spider, just as in most spider species, the female is larger than the male. Males approach females cautiously when attempting to mate, according to the biology department at the University of Wisconsin-La Crosse . Males perform a dance to get females' attention, and males often fight each other over the female. The female can be picky, and she often turns down many males before choosing a mating partner. Once she does pick one, the male needs to watch out; females often attack the males once copulation is finished.

The female then can store the sperm in a separate chamber from the eggs until she is ready to fertilize them. She will lay up to 1,000 eggs at a time, which are kept safe in a spun-silk egg sac.

Brazilian wandering spiders typically live for one or two years.

Brazilian wandering spiders' venom is a complex cocktail of toxins, proteins and peptides, according to the Natural History Museum in Karlsruhe, Germany. The venom affects ion channels and chemical receptors in victims' neuromuscular systems.

After a human is bitten by one of these spiders, he or she may experience initial symptoms such as severe burning pain at the site of the bite, sweating and goosebumps, Sewlal said. Within 30 minutes, symptoms become systemic and include high or low blood pressure , fast or a slow heart rate , nausea, abdominal cramping, hypothermia, vertigo, blurred vision, convulsions and excessive sweating associated with shock. People who are bitten by a Brazilian wandering spider should seek medical attention immediately.

Their  venom is perhaps most famous for triggering painful and long-lasting erections . For that reason, in a 2023 study, scientists reported that they were testing the venom in humans as a potential treatment for erectile dysfunction in those for whom Viagra didn't work.

However, these bites are rare, and envenomations, or exposure to these toxins from a spider bite, are usually mild, Vetter said. For instance, a 2000 study in the journal Revista do Instituto de Medicina Tropical de São Paulo found that only 2.3% of people with bites who came to a Brazilian hospital over a 13-year period were treated with antivenom. (The other bites did not contain enough venom to require it.) Most of the bites were from the species P. nigriventer and P. keyserlingi in eastern coastal Brazil. About 4,000 bites reportedly happen each year in Brazil, but only 0.5% of those cases are severe, according to a 2018 study in the journal Clinical Toxinology in Australia, Europe, and Americas . Meanwhile, 15 deaths have been attributed to Phoneutria in Brazil since 1903, the 2018 study reported. 

"It is unlikely that the spider would inject all of its venom into you, as this venom is not only needed as a means of defense but to immobilize prey," Sewlal said. "So if it did inject all of its venom, it [would] have to wait until its body manufactured more before it could hunt." That would also leave the spider vulnerable to being attacked by predators.

Furthermore, Sewlal pointed out that venom production requires a lot of a spider's resources and time. "So if the spider were to attack frequently and use up all of its venom, it [would] be safe to assume that it has a ready food supply to replace the energy and resources used. This situation does not exist in the wild."

• Learn more about Brazilian wandering spiders from the University of Wisconsin-La Crosse .
• Read about several species of Brazilian wandering spiders, including several images of the arachnids at the University of Florida .
• Find a spider in your bananas? It may or may not be a deadly species, according to the University of California, Riverside .

This article was originally published on Nov. 20, 2014. 

Sign up for the Live Science daily newsletter now

Get the world’s most fascinating discoveries delivered straight to your inbox.

Jessie Szalay is a contributing writer to FSR Magazine. Prior to writing for Live Science, she was an editor at Living Social. She holds an MFA in nonfiction writing from George Mason University and a bachelor's degree in sociology from Kenyon College. 

• Laura Geggel Editor

Giant, invasive Joro spiders with 6-foot webs could be poised to take over US cities, scientists warn

Diving bell spider: The only aquatic arachnid that creates a web underwater to live in

Lasers reveal prehistoric Irish monuments that may have been 'pathways for the dead'

Most Popular

• 2 Giant, 82-foot lizard fish discovered on UK beach could be largest marine reptile ever found
• 3 Global 'time signals' subtly shifted as the total solar eclipse reshaped Earth's upper atmosphere, new data shows
• 4 'I nearly fell out of my chair': 1,800-year-old mini portrait of Alexander the Great found in a field in Denmark
• 5 NASA reveals 'glass-smooth lake of cooling lava' on surface of Jupiter's moon Io
• 2 DNA analysis spanning 9 generations of people reveals marriage practices of mysterious warrior culture
• 3 5 catastrophic megathrust earthquakes led to the demise of the pre-Aztec city of Teotihuacan, new study suggests
• 4 'We were in disbelief': Antarctica is behaving in a way we've never seen before. Can it recover?
• 5 China green-lights mass production of autonomous flying taxis — with commercial flights set for 2025

Animal Corner

Discover the many amazing animals that live on our planet.

Brazilian Wandering Spider

The Brazilian Wandering Spider (Phoneutria fera) is an aggressive and highly venomous spider . It was first discovered in Brazil hence its name. However, this genus is known to exist elsewhere in South and Central America .

The Brazilian Wandering spider is a member of the Ctenidae family of wandering spiders.

The Brazilian Wandering spider appeared in the Guinness Book of World Records 2007 for being the most venomous animal .

In this particular genus, there are five known similar species whose members are also highly venomous. They include some of the relatively few species of spiders that present a threat to human beings.

Brazilian Wandering Spider Characteristics

The Brazilian wandering spider can grow to have a leg span of up to 4 – 5 inches. They are large hairy spindly-looking spiders who have eight eyes, two of which are large. Brazilian wandering spiders are fast-moving spiders, their legs are strong and spiny and they have distinctive red jaws which they display when angered.

The Brazilian wandering spider is not a Tarantula . Brazilian wandering spiders are not even in the same family group. Tarantulas are harmless to humans and are mostly ambush killers who wait for prey to come to them. Brazilian wandering spiders are active hunters. Brazilian wandering spiders and Tarantulas do have one thing in common, however, they do not eat bananas.

Brazilian Wandering Spider Habitat and Spider Webs

The Brazilian Wandering spider is so-called because it wanders the jungle floor, rather than residing in a lair or maintaining a web. This is another reason it is considered so dangerous. In densely populated areas, the Brazilian Wandering spider will usually search for cover and dark places to hide during daytime, leading it to hide within houses, clothes, cars, boots, boxes and log piles. This usually causes accidents when people disturb them.

The Brazilian Wandering spider is also called the ‘banana spider’ as it is occasionally found within shipments of bananas. As a result, any large spider appearing in a bunch of bananas should be treated with due care.

Brazilian Wandering Spider Diet

Adult Brazilian Wandering spiders eat crickets, other large insects, small lizards and mice. Spiderlings of this species eat flightless fruit flies and pinhead crickets.

Brazilian Wandering Spider Reproduction

All spiders produce silk, a thin, strong protein strand extruded by the spider from spinnerets most commonly found on the end of the abdomen. Many species use it to trap insects in webs, although there are many species that hunt freely such as the Brazilian Wandering spider. Silk can be used to aid in climbing, form smooth walls for burrows, build egg sacs, wrap prey and temporarily hold sperm, among other applications.

Brazilian Wandering spiders reproduce by means of eggs, which are packed into silk bundles called egg sacs. The male spider must (in most cases) make a timely departure after mating to escape before the females normal predatory instincts return.

Mature male spiders have swollen bulbs on the end of their palps for this purpose and this is a useful way to identify whether the spider is male or female. Once the sperm is inside the female spider, she stores it in a chamber and only uses it during the egg-laying process, when the eggs come into contact with the male sperm for the first time and are fertilized. The Brazilian Wandering spiders life cycle is 1 – 2 years.

Brazilian Wandering Spider Venom

Bites from the Brazilian Wandering spider may result in only a couple of painful pinpricks to full-blown envenomed. In either case, people bitten by this spider or any Ctenid should seek immediate emergency treatment as the venom is possibly life threatening.

The Phoneutria fera and Phoneutria nigriventer (two species of wandering spider) are the two most commonly implicated as the most vicious and deadly of the Phoneutria spiders.

The Phoneutria not only has a potent neurotoxin, but is reported to have one of the most excruciatingly painful envenoms of all spiders due to its high concentration of serotonin. They have the most active venom of any living spiders.

One of their members, the Brazilian Huntsman, is thought to be the most venomous spider in the world. Brazilian wandering spiders are certainly dangerous and bite more people than any other spiders.

Check out more  animals that begin with the letter B

More Fascinating Animals to Learn About

About joanne spencer.

I've always been passionate about animals which led me to a career in training and behaviour. As an animal professional I'm committed to improving relationships between people and animals to bring them more happiness.

Fact Animal

Facts About Animals

Brazilian Wandering Spider Facts

Brazilian wandering spider profile.

There are more than 50,000 species of spider, and the vast majority are less dangerous than a honeybee. Almost none are aggressive, and of those with medically significant venom, only a small percentage are capable of causing death. So, on the whole, arachnophobes are just being a bit silly.

But there’s one spider that vindicates all of these fears, and few animals are as globally renowned to be a serious threat to human lives as the Brazilian Wandering Spider .

Brazilian Wandering Spiders are actually 9 species of spider in the same genus ‘Phoneutria’, one of which is found in Central America, with the rest in South America.

Brazilian Wandering Spider Facts Overview

These spiders are called wandering spiders because of instead of spinning a web to wait for food, or occupying a lair, they spend their night wandering in the leaf litter of the jungle floor for prey.

The sensitive hairs on its body help detect vibrations of passing prey, and it will feed on insects, lizards, frogs and any animals as large as itself.

During the day they will hide under logs, rocks, or inside termite mounds and banana plants. They will also sometimes wander into urban areas and homes, where they can come into contact with humans.

Brazilian wandering spiders are aggressive , dangerous and frightening. For once, this is an animal you should be wary of.

The females are larger, around 50% heavier than males, and produce more venom, and this might be a clue as to why their Greek name translates to “ Mudress” . These spiders will often stand and fight and have an intimidating threat display.

The potency of their venom is one of the reasons they’re so dangerous, and their ability to hide away in fruit and shoes explains why most bites are on extremities.

Interesting Brazilian Wandering Spider Facts

1. armed spiders.

In Brazilian, these are sometimes known as armed spiders, on account of their elongated front legs.

They can convey quite a bit of information with these legs, and as wandering spiders, use them to get about the forest, looking for food.

2. Banana Spiders

They’re also sometimes called ‘banana spiders’ on account of their status as a stowaway on popular fruit imported from the tropics.

This is becoming less common as stricter regulations ensure there’s less contamination of fruits, but there’s always a chance your next bunch of bananas will have a family of these spiders living inside it.

3. They have the largest venom glands of any spider

Females produce more venom than males, but both sexes have enormous venom glands. These glands are even more impressive when you consider the size of the spider is significantly less than the largest around.

The venom glands of the Brazilian Wandering Spider are over a centimetre long, and this is all housed inside the bright red chelicerae (mouth parts) which they are quick to display whenever they get upset. 1

4. They’re aggressive

These spiders can grow quite large and have long, brightly-coloured legs. Unlike most spiders, they’re known to stand their ground when threatened and are far quicker to bite than many other species.

They’ll still try to scurry away where possible, and they’re not out to get anybody.

But where most other species will flee, the wandering spiders’ aggression does make it more likely to be involved in incidents.

Most bites are on fingers and toes, a sign that they’re being stepped on or grabbed inadvertently. When the spider feels cornered, it’ll rear up on its back legs and waves its colourful arms around as a warning.

Then it’ll sway side to side, beckoning you to have a go. Anything foolhardy enough to call this bluff gets a wealth of envenomation effects. 2 3

5. They give some men erections

There are ways to accomplish this with fewer side effects, but a bit from a Brazilian wandering spider does come with a certain Viagral quality.

This isn’t as fun as it might sound. Prolonged erections in this manner are likely to harm and destroy muscles and blood vessels in the penis and could cause irreparable damage.

Besides this, the assault on the central nervous system that comes with envenomation by this spider doesn’t sound worth it. 4

6. And some people die

This assault brings with it a whole host of unpleasant symptoms. Seizures, foaming at the mouth, inability to speak, collapse, and a host of other miserable experiences.

Paralysis is possible, as is cardiac shock. Blood vessels can burst in the brain, or anywhere else, and in many cases, this can be enough to kill a person.

This spider has one of the most potent venoms of all, and there are multiple legitimate records of death as a result of bites.

7. But they’re rarely fatal

While the Brazilian wandering spider is potentially one of the most dangerous spiders in the world, there is some evidence to suggest it gives a dry bite, defensively.

This means that despite exceptionally toxic venom, the amount actually injected is less than some of the other contenders, and this is what makes it typically less lethal than the Australian funnel webs.

These spiders are classified as Dangerous Wild Animals and would therefore require a special permit to keep. Bites from wandering spiders are common in South America, but antivenom is often readily available, and they rarely result in death.

In most cases, lethal bites are cases of a very young or very old victim, and few people of healthy age are killed. 5

8. They do invade the UK sometimes

These unquestionably scary spiders show up in supermarkets in the UK on occasion, having hitched a ride on banana shipments.

On more than one occasion they’ve made their way into shoppers’ homes, but it doesn’t appear that there are any cases of them biting people as a result.

These spiders aren’t suited for temperate climates and don’t survive Winter, so there’s no risk of them multiplying.

Brazilian Wandering Spider Fact-File Summary

Scientific classification, fact sources & references.

• PeerJ. (2017), “ Dimensions of venom gland of largest venom glands in all spiders ”, Bio Numbers.
• Dave Clarke (2010), “ Venomous spider found in Waitrose shopping ‘beautiful but aggressive’” , The Guardian.
• “ Phoneutria Perty (Arachnida: Araneae: Ctenidae) ”, UF-IFAS University of Florida
• Kátia R.M. Leite (2012), “ Phoneutria nigriventer spider toxin Tx2-6 causes priapism and death: A histopathological investigation in mice ”, Science Direct.
• “ Brazilian wandering spiders: Bites & other facts ”, Live Science.

Brazilian Wandering Spider: Care, Food, Habitat & Preventions

Mike Wallace

Have you ever heard of or do you know what a  Brazilian wandering spider is ? It is a big venomous spider from places like Central and South America, and people sometimes call it the  banana spider . Why? Well, we are about to find out!

Table of Contents

These wandering spiders are aggressive hunters who go out on the hunt at night. Their meals include both invertebrates (like insects) and vertebrates (creatures with a backbone, like small animals).

These spiders are super dangerous because their venom is like a powerful potion that can make people really sick or even worse. They usually hang out in tropical rainforests and even in cities, hiding in banana plants. 

So, let’s get more information about the world of this sneaky spider to learn the details about its looks, eating habits, where it lives, the venom it carries, and find out if it is genuinely risky. Ready to explore? Keep reading!

Brazilian Wandering Spider Description:

Scientific name and family:.

In Brazil, they are sometimes known as “ armed spiders ” (armadeiras), and they share the name “ banana spiders ” with a few other spiders. They have different names, but they are all talking about the same interesting spider!

The Brazilian wandering spider, scientifically known as  Phoneutria , Maximilian Perty kickstarted the Phoneutria genus in 1833. The name comes from the Greek word φονεύτρια , which means “murderess” and falls under the Animalia kingdom, Arthropoda phylum, and Arachnida class.

Within Arachnida, it is classified in the order Araneae, infraorder Araneomorphae, and Ctenidae family. The genus Phoneutria, described by Perty in 1833, includes the type species  Phoneutria fera .

This classification helps us understand where these spiders fit into the larger picture of living organisms.

The following 9 species are accepted by The  World Spider Catalog :

• Phoneutria bahiensis
• Phoneutria boliviensis
• Phoneutria eickstedtae
• Phoneutria fera
• Phoneutria keyserlingi
• Phoneutria nigriventer
• Phoneutria pertyi
• Phoneutria reidyi
• Phoneutria depilata

What do Brazilian Wandering Spider look like?

Size range:.

The spiders in the Phoneutria group can get pretty big in size. Their legs can stretch out to be 13 to 18 centimeters (5 to 7 inches) wide, and their bodies can have a range between 17 to 48 millimeters (a little more than half an inch to almost 2 inches) long.

The female Brazilian spiders can get pretty big, reaching up to 15 centimeters (5.9 inches) in length. On the other hand, the males are smaller, usually measuring around 7 centimeters (2.8 inches). They usually weigh up to 0.21 ounces.

They have long, slender legs, and even though some other spiders with different names might have longer legs, the Phoneutria spiders are champions when it comes to having the longest bodies and being the heaviest in their spider gang.

The spider’s body has two main parts. The first is the prosoma, kind of like its “head,” where you will find all eight legs, eyes, fangs (chelicera), and little multitasking arms (pedipalps).

The second part is the opisthosoma, holding the spinnerets for making silk, the back end opening (anal opening), “the lungs,” the heart, and the important bits for making baby spiders (reproductive organs).

So, the prosoma is like the front control center, and the opisthosoma is like the back office, handling things like silk-making and baby-making.

Brazilian spiders come in different colors, with most being hairy and shades of brown and gray. Some species may have lightly colored spots on their abdomen.

A distinctive feature of many species is the presence of bands of black and yellow or white on the underside of their two front legs.

Identification:

To identify a spider from the Phoneutria group, look for a dense brush of fine hairs on their leg parts. They might seem like other spiders, especially  Cupiennius , but here is how you can differentiate: 

• Phoneutria often have a dark line on the front of their palps and a thin black line on top of their head. 
• Check underneath, too; their legs usually have dark parts and light joints. Sometimes, the belly has black dots or is reddish. 
• Usually it has been observed that when they are upset, they do a cool defensive move like lifting their front legs high with a distinctive pattern. So, if you see a spider doing that dance, it is probably a Phoneutria!

Brazilian Wandering Spiders live all over the Americas, from Costa Rica to northern Argentina. They are like the residents of the jungle, chilling in forests east of the Andes in countries like Colombia, Venezuela, Ecuador, Peru, Bolivia, Brazil, Paraguay, and the Guianas.

Some, like P. reidyi, P. boliviensis, and P. fera, love the Amazon rainforest, while others prefer the Atlantic Forest in Argentina, Paraguay, and Brazil.

They have also made themselves at home in the Cerrado savanna. But if you head to northeastern Brazil, they are not around. These spiders have even taken trips to Chile and Uruguay.

Why are they called Banana Spiders?

These spiders are linked with bananas. Richard S. Vetter, a researcher at the University of California, found that these powerful spiders sometimes end up in North America and Europe by accident, hitching a ride in banana shipments.

But it is often a case of mistaken identity. Only a few Phoneutria species have been found in banana shipments, and sometimes, other spiders get the blame due to misidentification. 

What They Like to Eat or Hunt?

Their food includes flies, beetles, butterflies, moths, grasshoppers, locusts, and crickets. Occasionally, they might even feast on small creatures like amphibians, reptiles, or mice. All these diet or food findings tell us about how diversified eating habits these fascinating spiders have.

Mating and Lifecycle:

Like most spiders, the female spiders are bigger than the males. When the male spider wants to be friends, they do a little dance (vibrating his pedipalps and specialized sensory appendages) to signal his intentions to impress the female, but it is a cautious approach.

The behavior of the female can be choosy, and she might say no to a few before picking the right one. 

After the dance, sometimes, the females decide to attack them, or if she is interested, she can store the male’s baby-making material in a special place until she is ready to use it.

Then, she lays a bunch of eggs, up to 1,000 at a time, and keeps them safe in a silk egg sac. Sadly, after laying her eggs, the mom spider says goodbye. It is her way of making sure the new spiders are ready to explore the world on their own.

The lifespan of the banana spider (Phoneutria nigriventer) differs for males and females. Females usually live for 6 to 8 weeks after reaching maturity, while males have a shorter lifespan of 2 to 3 weeks after their last molt. 

Certain mammals, like coatis (Procyonidae, which includes raccoons) and other small insectivores, birds are potential predators of large wandering spiders.

These spiders got their name as wandering spiders because of the fact that they are not into web building. Instead, they stroll around the forest floor at night(nocturnal), searching for dinner.

Brazilian Wandering Spiders are active hunters and use both ambush tactics and direct attacks to catch their prey. During the day, they prefer cozy spots like under logs or in crevices, only emerging at night for their hunting adventures. These spiders do not build nests like other spider species.

While wandering spiders are not naturally aggressive towards humans, they won’t hesitate to bite if they feel cornered or threatened. Most bites happen when a spider accidentally gets trapped in clothing or bedding. 

Bite and Venom:

The bite of the armed spider is the most dangerous in the world as the venom it carries can be harmful to humans.

The danger is not just about how strong the venom is; it is also about factors like the spider’s likelihood to bite and how close it is to where people live.

These spiders often hide in houses, clothes, and other dark places during the day, making accidental bites more likely. 

While their fangs are adapted for small prey, some experts think they might give a “dry” bite in defense to save venom. Studies suggest that not all bites inject venom, and serious cases requiring antivenom are rare.

However, there have been confirmed cases of death, with symptoms appearing quickly, including:

• Severe pain
• Breathing difficulties
• Increased heart rate and blood pressure
• In severe cases, paralysis and death

The severity can depend on the spider’s sex, with females generally more dangerous. The spiders produce less venom in colder months, and a small amount can be potent enough to harm.

Fortunately, bites from Brazilian spiders are rare, and when they do occur, the exposure to the toxins is generally mild, as explained by Vetter.

Also Read: What is a Huntsman Spider? (Heteropodidae) – The Ultimate Guide

Banana Spider’s Facts:

Below are essential details about Brazilian wandering spiders:

• They hold the title for the world’s largest spiders , boasting leg spans reaching up to 15 centimeters (6 inches).
• Their venom packs a powerful punch, capable of inducing severe pain, paralysis, and, in extreme cases, fatal outcomes for humans.
• Despite their intimidating reputation, they are generally non-aggressive and resort to biting only when provoked.
• These spiders inhabit tropical rainforests and urban areas across Central and South America.
• In case someone has been bitten by this spider, he/she needs quick medical treatment to control the effects timely.

Treatment and Preventive Measures:

If bitten by a wandering spider or armed spiders, prompt medical attention is crucial. There is an antivenom for the spider’s venom, but its effectiveness is highest when administered within a few hours of the bite.

To prevent a bite:

• Wear protective clothing, use shoes and long pants when in areas where these spiders are found.
• Before wearing your clothes and shoes, make sure to check them to ensure no spiders are hiding.
• Maintain cleanliness and avoid leaving food or garbage exposed, as this can attract spiders.

These preventive measures are essential for minimizing the risk of encountering and getting bitten by Banana spiders.

Can Brazilian spiders kill humans?

Brazilian wandering spiders (Phoneutria nigriventer) are venomous and can potentially kill a human with a single bite. Their venom contains a potent neurotoxin that can cause severe pain, paralysis, and even death. 

Are Brazilian spiders poisonous?

Yes the venom of this spider is poisonous, that can cause death. While Brazilian wandering spiders are potentially dangerous, actual bites are relatively rare.

By adopting preventive measures and promptly seeking medical attention if bitten, the risk of serious complications can be significantly reduced.

Can you keep Brazilian spiders as pets?

It is strongly advised against keeping wandering spiders as pets due to their venomous nature and the potential risk to human safety.

Managing these spiders in captivity demands specialized knowledge and handling procedures to minimize the risk of bites.

Final Thoughts:

The Brazilian wandering spider, banana spider, or armed spider is a large and venomous arachnid found in Central and South America. While their potent venom can be harmful to humans, encounters are rare.

These nocturnal hunters have adopted various habitats, from rainforests to urban areas, and are associated with banana shipments. Understanding their appearance, behavior, and habitat is crucial for minimizing risks.

Seeking immediate medical attention after a bite is essential, as antivenom is available but most effective when administered promptly. Despite their fearsome reputation, the Brazilian spider remains a captivating and potentially dangerous species.

About the author

Spiders Planet is the ultimate source of information about spiders! All the articles on this site will provide you with the most accurate and up-to-date information written by me and a group of writers, that is well-researched and fact-checked before it’s published.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Save my name, email, and website in this browser for the next time I comment.

Latest posts

Do Spiders Eat Fruit? Unveiling the Secrets

The connection between animals and fruits showcases a captivating example of mutualism, a win-win relationship where both parties benefit. Animals gain access to…

Are Woodlouse Spiders Venomous?

The Woodlouse spider (Dysdera crocata) is a spider species that mainly hunts and feeds on woodlice and is mostly found in Africa, Asia,…

What Does Wolf Spiders Eat?

Wolf spiders are excellent hunters of small insects. Unlike spiders that weave intricate webs, these predators rely on sharp vision and stealthy moves…

The World’s Most Venomous Spider: The Brazilian Wandering Spider

Published: August 10, 2023

• Facebook 19

Few things fear people’s hearts, like the mention of venomous spiders. According to the Guinness World Records , venomous spiders are some of the most dangerous creatures on Earth, and the Brazilian Wandering Spider is the world’s most venomous spider. This elusive arachnid is found in the rainforests of South America, more specifically in Brazil, Argentina, and French Guiana. ‘

It is considered one of Brazil’s most significant public health threats, with a potent venom capable of causing extreme pain, paralysis, and even death. Despite its fearsome reputation, however, the Brazilian Wandering Spider is a fascinating and unique creature with many incredible features that make it stand out from other spiders.

Jump ahead to any section below!

Brazilian Wandering Spider’s Habitat And Range

The Brazilian Wandering Spider is found in the rainforests of South America. These rainforests are the world’s largest and most biodiverse terrestrial ecosystems, covering an area of approximately 6.7 million square kilometers. This equates to roughly 40% of the total area of South America and spans nine countries, including Brazil, Peru, and Colombia.

The Amazon rainforest, the most extensive rainforest on Earth, plays an essential role in the Brazilian Wandering Spider’s habitat and range. The spider relies heavily on the dense canopy of trees in these rainforests for shelter and protection. The Amazon rainforest also provides the spider with an abundant prey source, including insects, small mammals, and other spiders.

The South American rainforests are characterized by their warm and humid climate, which creates the ideal conditions for the Brazilian Wandering Spider to thrive. These ecosystems also have a high rainfall throughout the year, which sustains the dense vegetation and creates an ideal environment for spiders to hunt and reproduce.

Check out: Uncovering The Enigma Of The Greenland Shark .

Specific Locations The Brazilian Wandering Spider Is found

The Brazilian Wandering Spider is most commonly found in Brazil. It can also be found in other parts of South America, such as Argentina and French Guiana. In Brazil, the spider can be found in various regions. This includes the Amazon rainforest, the Atlantic forest, and the Caatinga ecosystem.

The Atlantic forest, a tropical forest that spans the Brazilian coast, is also an important habitat for the Brazilian Wandering Spider. This ecosystem is under threat due to deforestation and urbanization. This has resulted in the loss of the spider’s natural habitat.

In French Guiana, the spider is present in the Guiana Amazonian Park, a protected area of the Amazon rainforest managed by the French government. The Guiana Amazonian Park is home to an array of biodiversity, including monkeys, jaguars, and over 400 species of birds. This makes it an important region for the Brazilian Wandering Spider’s survival.

Brazilian Wandering Spider’s Characteristics

It is a remarkable arachnid species found in the rainforests of South America. It has numerous unique characteristics from other spiders. The Spider is large and intimidating, measuring up to 10 inches (25 cm) in leg span. Its body size ranges from 0.98 to 1.77 inches (2.5 to 4.5 cm) in length. This makes it one of the biggest spider species in the world. It has eight long and hairy legs, which help it move quickly and climb trees effortlessly.

The spider is striking, with a shiny brown or black body covered in short fine hairs. Its legs are also covered in fine hairs with dark stripes or spots. These markings have a unique pattern that helps to identify the spider from other species. Unlike most spiders, the Brazilian Wandering Spider doesn’t have a distinct web-spinning organ. But instead moves around the forest floor and trees in search of its prey.

Behavioral Patterns

They are aptly named because they are known to wander about and can cover a great distance while searching for food and mates. It’s primarily active at night and is a solitary hunter that preys on insects, spiders, rodents, and even small snakes. It has a unique hunting technique where it grabs its prey with its front legs and sinks fangs into it, injecting a potent venom that can quickly immobilize and kill its victim. Interestingly, this spider can adjust the amount of venom it injects to fit the size and the species of its prey, a skill unique to this spider species.

During the mating season, the Brazilian Wandering Spider’s behavior changes drastically. The males will approach females cautiously, tapping on the female’s web to signal their interest. They then perform a complex courtship display that involves waving their front legs, drumming their pedipalps, and vibrating on webs. The more intricate the display, the higher the chances of mating with the female.

Brazilian Wandering Spider’s Venom

The venom of the Brazilian Wandering Spider is considered one of the deadliest in the world. The potency of the venom is due to the presence of a potent neurotoxin called PhTx3. This toxin is responsible for the spider’s ability to paralyze its prey and defend itself from predators.

In scientific studies, the venom of the Brazilian Wandering Spider has proven to be significantly more toxic than other venomous spiders, such as the Black Widow and the Brown Recluse. The effectiveness of this venom is a result of its unique chemical structure, which allows it to attack the nervous system of its victim and cause significant damage.

Check out: Bengal Tiger Vs. Western Diamondback Rattlesnake .

Effects Of Venom On Humans And Animals

The effects of the venom of the Brazilian Wandering Spider can be devastating. When bitten, victims may experience severe pain, muscle spasms, and even paralysis. In extreme cases, the venom can cause respiratory failure, resulting in death.

While the venom initially causes pain and discomfort, it can lead to severe, long-lasting symptoms. In addition to causing physical harm, the venom can also have psychological effects, leading to anxiety, panic attacks, and even PTSD.

Animals are also susceptible to the venom of the Brazilian Wandering Spider . Small animals, such as mice and birds, are often preyed upon by the spider, while larger animals, such as dogs and cats, can be bitten accidentally.

Public Health Threat And Prevention

The Brazilian Wandering Spider is a significant public health threat in Brazil, with hundreds of bites reported yearly. The spider has a wide distribution throughout the country, and its venom is particularly toxic, making it a serious risk to human health.

Also, the spider is especially dangerous because it is highly aggressive and can be found in homes, gardens, and public spaces. This means that anyone can encounter the spider, regardless of location or occupation, making it difficult to prevent bites.

Measures Taken To Prevent Bites And Treat Victims

To prevent bites from the Brazilian Wandering Spider, it is essential to take steps to reduce the risk of encountering the spider. This may include keeping homes and gardens free of clutter and debris, sealing windows and doors, and avoiding areas where the spider is known to live.

In the case of a bite, it is important to seek medical attention immediately. While no anti-venom is available, medical professionals can offer supportive care to alleviate symptoms and prevent complications.

Frequently Asked Questions

The Brazilian Wandering Spider’s venom contains a potent neurotoxin called PhTx3, which can cause extreme pain, muscle spasms, and paralysis in humans. This venom is 20 times more deadly than a rattlesnake’s and can cause respiratory failure in victims.

It is primarily found in South and Central America, especially in the Amazon rainforest. They hide in dark, moist environments such as piles of leaves, tree trunks, and burrows.

To avoid contact with the Brazilian Wandering Spider, it is important to inspect your surroundings, especially before putting on shoes or reaching into piles of debris. It is advisable to wear gloves and long sleeves when working outdoors or in areas where these spiders may be present. If you suspect a Brazilian Wandering Spider has bitten you, seek medical attention immediately.

Overall, the Brazilian Wandering Spider is a fascinating and deadly spider that deserves respect and caution. Though its venom is incredibly potent, the spider itself is a remarkable creature. Its ability to move quickly and seamlessly across the ground makes it a formidable predator in its native habitats. 

Despite its fearsome reputation, we must learn to coexist with this remarkable arachnid, preserving its unique place in the natural world. Given the immense diversity and beauty of our planet’s biodiversity, the Brazilian Wandering Spider reminds us that we still have much to learn about the remarkable creatures that share our planet.

thanks for reading along! See below for related article links.

• Basking Shark
• Whale Sharks
• Tiger Shark Encounters
• The Great White Shark: Apex Predator of the Ocean
• Gorilla And Anaconda In Focus
• The English Bulldog
• Largest Asian Elephant
• The Largest Gorilla Ever Recorded
• Latest Posts

• Watch Starfish Walking on the Beach - April 24, 2024
• Watch Bison Stampede Around Bus in Yellowstone - April 24, 2024
• How to Survive a Tiger Attack - April 23, 2024

• Orb Weavers
• Jumping Spiders
• Cobweb Spiders
• Huntsman Spiders
• Wolf Spiders
• Sicariidae Spiders
• Funnel Weaver Spiders
• Crab Spiders
• Nursery Web Spiders
• Spiders in US
• Parts of a Spider
• Life Cycle of a Spider
• Where do Spiders Live
• What do Spiders Eat
• How long do Spiders Live
• Where do Spiders go in Winter
• Spider Ballooning
• Spider Molting
• Spider Eyes
• Spider Predators

Brazilian Wandering (Phoneutria)

The Brazilian Wandering spider is a type of spider from the Phoneutria group. Even though they’re called “Brazilian,” not all of them come from Brazil. They’re known for having strong venom. In this post, we’ll share cool facts about these spiders and help you understand them better.

Scientific Classification

• Family: Ctenidae
• Genus: Phoneutria

List of Spiders Belonging to This Genus

Physical description and identification.

• Size: They are large in size, with their body being 17- 48mm (.67 – 1.89 inches) long and they also have a leg span of 130 – 150 mm (5.1-5.9 inches).
• Color: The color may vary from one species to the other, though most of them have a brown hairy body, with black spots on their stomach. Some have bright, red hairs on their mouthparts or chelicerae, while others may lack it, a feature that confuses them with species of another genus, particularly the Cupiennius.
• Other characteristics: They often lift their body in an erect posture and hold their frontal legs high to defend themselves against predators.

They are safely placed in a silken sac and the female spiders of this genus are known to lay about 1000 of them in her lifetime.

Spiderlings

They remain with their mother for some time after which they disperse to be on their own. The juvenile spiders are known to consume pin crickets as well as non-volatile fruit flies for their diet.

The spiders of this genus do not build webs but walk on the jungle floor, on the lookout for their prey.

Are Brazilian Wandering Spiders Venomous?

Yes, Brazilian Wandering Spiders have strong venom. They use it to catch their food. It’s one of the reasons they’re pretty famous.

Can Brazilian Wandering Spiders Bite?

Yes, they can bite. While they don’t always want to, they might if they feel scared or threatened by something.

How Fast Can a Brazilian Wandering Spider Kill One?

It has been reported that the bite of species belonging to this genus may result in the victim’s death within one hour after the venom enters the person’s body. However, with effective anti-venom being introduced for treatment in Brazil to combat the toxic effects of these spiders, the incidence of fatalities has been less. In fact, most studies show that death mostly occurs in children below seven years of age. Of all the eight species, P. nigriventer , followed by P. fera, is said to account for most venom intoxications in Brazil.

Ecological Importance and Behavior of the Brazilian Wandering Spider

The Brazilian Wandering Spider, scientifically known as Phoneutria, stands out not just because of its reputation as one of the world’s most venomous spiders , but also due to its ecological significance and unique behavior.

Natural Predator: Despite their fearsome reputation, Brazilian Wandering Spiders are not at the top of the food chain. They fall prey to larger animals and birds. Among their predators are the coatis, certain species of large spiders, and a variety of avian predators.

Prey-Predator Dynamics: The dynamic between the Brazilian Wandering Spider and its prey is a showcase of nature’s balance. While they are efficient hunters, specializing in ambushing their prey, their own survival is constantly under threat from their predators. This cycle ensures that no one species dominates the ecosystem and that biodiversity thrives.

Relationship with Humans: The relationship between humans and the Brazilian Wandering Spider is one of respect and caution. Their venom is potent and can be harmful to humans, although fatal encounters are rare. 

Quick Facts

Did You Know

• Species of this genus are known for wandering along the jungle floor during the night which is why they are referred to as “wandering spiders”.
• They are alternately called “banana spiders”, a name that they also share with other species because members of this genus have often been found in banana shipments. Research in shipments going to North America showed that 7 of the 135 spiders found in such shipments were of the Phoneutria genus.
• Phoneutria in Greek means murderess, a name perfectly attributed to its aggressive nature.

Other Spiders in this Family

Wandering spiders.

Phoneutria depilata

Phoneutria boliviensis

Phoneutria fera

Phoneutria nigriventer

Southeastern Wandering (Anahita punctulata)

• Terms and Conditions
• Privacy Policy

Brazilian Wandering Spider

The Brazilian Wandering Spider (Phoneutria fera) is a teardrop-shaped arachnid with a brown coloration. Known for its potent venom, it thrives in both the lush rainforests and human dwellings of Brazil. Its notorious wandering behavior makes it a significant presence in its habitats.

Fascinating Facts about Brazilian Wandering Spider

Here are 3 interesting facts about Brazilian Wandering Spider:

• The Brazilian Wandering Spider is considered the world's most venomous spider by the Guinness World Records.
• They are known as 'wandering' spiders because they roam the jungle floor at night instead of residing in a lair or web.
• Despite their notorious reputation, their bites rarely cause death in humans due to the small amount of venom they inject.

Taxonomy and Classification

Here is the scientific categorization of Brazilian Wandering Spider, providing a glimpse of their position in the biological hierarchy:

Lifecycle and Growth

Brazilian Wandering Spider's life is a journey of transformation - an adventure marked by the following captivating stages:

Egg → Spiderling → Adult

The Brazilian Wandering Spider, primarily found in the rainforest, exhibits a lifecycle that spans both wilderness and human habitats. From egg to adult, it navigates a complex path through dense foliage and human dwellings, adapting to these contrasting environments.

Brazilian Wandering Spider Behaviour and Adaptations

Brazilian Wandering Spiders are known for their nomadic behavior. Instead of building webs to catch prey, they actively hunt at night, using their highly developed senses, particularly vision, to locate and stalk their prey.

These arachnids have adapted to a wide range of habitats, from forests to urban areas. Their potent neurotoxic venom, one of the most powerful among spiders, allows them to incapacitate and consume a variety of prey.

Brazilian Wandering Spider Interaction with the Ecosystem

Now, let's look at how they help maintain the balance in the ecosystem:

• Brazilian Wandering Spiders play a crucial role in controlling the population of their prey, which includes insects and small mammals.
• They serve as a food source for larger animals, contributing to the food chain in their ecosystem.
• Their venom, although dangerous to humans, is studied for medicinal purposes including treatments for erectile dysfunction and pain relief.

Threats to Brazilian Wandering Spider

Despite their popularity and predator status, Brazilian Wandering Spider encounter several threats as well:

• Destruction of natural habitat due to deforestation
• Increased usage of pesticides affecting their food chain
• Climate change potentially disrupting their breeding patterns

Also Check:

If you're interested in learning about at some more interesting insects, here are two suggestions for you, below 👇

Do Brazilian Wandering Spider Bite?

Learn if Brazilian Wandering Spider bite, what you should do if you get bitten by them, and other interesting information.

Other Bug Profiles

Check other interesting bugs:

• Lone Star Tick
• Brown Dog Tick

What do Brazilian Wandering Spider Eat?

Learn what food Brazilian Wandering Spider eat, and also information about how they eat and drink.

Wellcome to SpiderZoon

• Spider Facts

Brazilian Wandering Spider: Size, Bite, Diet and Other Facts 

The Brazilian Wandering Spider is a venomous arachnid with a fearsome reputation. This South American rainforest dweller packs a powerful punch, but don’t let its nickname “banana spider” fool you – they’re active hunters, not web-spinners. Despite their size and venom, bites are uncommon as they’re typically shy.

The Brazilian Wandering Spider has garnered significant attention due to its potent venom, which makes it one of the world’s most venomous spiders. Understanding its biology and behavior is crucial for both scientific research and public safety.

Table of Contents

Scientific classification

The Brazilian Wandering Spider belongs to the family Ctenidae within the order Araneae. Its scientific classification is as follows:

• Kingdom: Animalia
• Phylum: Arthropoda
• Class: Arachnida
• Order: Araneae
• Family: Ctenidae
• Genus: Phoneutria

The Brazilian Wandering Spider goes by various common names, including “armed spider,” “banana spider,” and “wandering spider.” Synonyms for this species may include Ctenus , which was formerly used for some Phoneutria species.

Distribution and habitat

Distribution:

• South American:  Found throughout most of South America east of the Andes mountains, including countries like Brazil (their namesake), Argentina, Paraguay, and up into northern regions.
• Central American Touch:  A few species even reach southern Central America.
• Rainforest Dwellers:  Primarily found in the lush rainforests of South America.
• Not Picky Places:  They can also adapt to other habitats like the Atlantic Forest and even some urban areas.
• Daytime Hideouts:  While they wander at night, they seek shelter during the day in places like termite mounds, under rocks, or even (unintentionally) in bananas!

Physical Characteristics

 size and weight.

The Brazilian wandering spider is a creepy crawly giant. Their bodies can grow up to 2 inches (5 centimeters) long, but that’s not the scary part. Their legs can span a whopping 7 inches (18 cm), making them look even bigger. They are one of the biggest true spiders by body weight and size.

Despite their impressive leg span, Brazilian wandering spiders are relatively light. They only weigh around 6 grams, which is about the same as two pennies. While they might look imposing, they’re not the heaviest arachnids around.

Coloration and markings

These spiders exhibit a range of colors, including brown, black, and sometimes reddish hues. They often have distinctive markings on their bodies, which can vary between species. These markings may include stripes or patterns that serve as a key identification feature.

Notable features

One of the most notable features of the Brazilian Wandering Spider is its elongated, robust body and long, agile legs. They have sharp fangs, which they use to inject venom into their prey or in self-defense.

Sexual dimorphism

Sexual dimorphism is evident in this species. Females are larger and bulkier than males, while males have longer, more slender legs. Additionally, males possess specialized structures known as pedipalps, which are used during mating.

In the following sections of this article, we will delve deeper into the behavior, venom, and ecological role of the Brazilian Wandering Spider, shedding light on why this species has both fascinated and instilled fear in those who encounter it.

 Behavior and Ecology

 Nocturnal habits

Brazilian Wandering Spiders are primarily nocturnal creatures. They are most active during the night, venturing out of their daytime hiding places to hunt for prey and engage in mating activities. Their nighttime activity allows them to avoid predators and reduce the risk of desiccation in the hot tropical sun.

Hunting and feeding behavior

These spiders are agile hunters and primarily feed on insects, small vertebrates, and other arachnids. They do not build webs to capture prey but instead rely on their excellent senses, including acute vision and touch, to locate and stalk their victims. They often wander in search of food and are known for their swift and lethal strikes. Once they subdue their prey, they inject venom to immobilize it before feeding.

Brazilian Wandering Spiders (Phoneutria) are active hunters and have distinctive feeding behaviors. Here’s an overview of their feeding habits:

• Active Predators: Brazilian Wandering Spiders are not web-builders like many other spider species. Instead, they are active predators. They actively roam their environment in search of prey rather than waiting for insects to stumble into a web.
• Hunting Strategy: When hunting, these spiders use their excellent senses, including acute vision and touch, to locate potential prey. They are known for their agility and speed, which they use to stalk and capture their victims. They have sharp fangs, which they use to deliver a venomous bite to immobilize their prey.
• Diet: Their diet consists primarily of insects and other arthropods, but they are opportunistic feeders and may consume a variety of small creatures, including small vertebrates such as frogs and lizards when the opportunity arises.
• Venom Use: Brazilian Wandering Spiders inject venom into their prey to immobilize and partially digest it. Their venom contains neurotoxins that affect the nervous system of their victims. Once the prey is incapacitated, the spider can feed on it at its leisure.
• Feeding Process: After subduing their prey with a venomous bite, the spider uses its chelicerae (fangs) to break down the prey’s tissues. The venom also helps in predigestion, turning the prey’s insides into a semi-liquid form that the spider can ingest. They can consume both the internal fluids and solid parts of their prey.
• Frequency of Feeding: The frequency of feeding can vary depending on factors such as the availability of prey and the spider’s size. Generally, they need to feed periodically to sustain their energy and growth. Spiderlings may require more frequent meals to support their rapid growth, while adults can go longer periods between meals.

Overall, the Brazilian Wandering Spider’s feeding strategy is well-suited for their active and wandering lifestyle, allowing them to efficiently capture and consume a variety of prey in their natural habitat.

Role in the ecosystem

The Brazilian Wandering Spider plays a vital role in controlling insect populations within its habitat. By preying on a variety of insects and other small creatures, they help maintain ecological balance. Additionally, their presence in the rainforest ecosystem contributes to the overall biodiversity and food web.

Mating and reproduction

Mating in Brazilian Wandering Spiders is a complex and potentially dangerous process. Male spiders must carefully approach and court a receptive female to avoid being mistaken for prey. They use specialized pedipalps to transfer sperm to the female’s reproductive organs. After successful mating, females lay egg sacs containing hundreds of eggs. They guard these sacs and ensure the survival of their offspring until they hatch.

 Lifespan and growth

The lifespan of Brazilian Wandering Spiders varies between males and females. Males generally have a shorter lifespan, typically living for a few months to a year after reaching maturity. Females, on the other hand, can live for several years. The growth of these spiders involves a series of molts, during which they shed their exoskeletons to accommodate their increasing size. Molting is a vulnerable period in their lives as their new exoskeleton is initially soft and requires time to harden.

Venom and Envenomation

Composition of venom.

The venom of Brazilian Wandering Spiders is a complex mixture of neurotoxins, cytotoxins, and other enzymes. One of the most significant components is a neurotoxin called PhTx3, which targets the nervous system of their prey.

Toxicity and effects on humans

The venom of these spiders is highly potent and can be lethal to their prey. In humans, envenomation can cause a range of symptoms, including intense pain, muscle cramps, fever, nausea, and in severe cases, paralysis and death. It’s important to note that while their venom is potent, actual fatalities from Brazilian Wandering Spider bites are rare due to the availability of medical treatment.

First aid and medical treatment

In the event of a Brazilian Wandering Spider bite, immediate medical attention is crucial. First aid measures may include cleaning the wound and applying ice to reduce pain and swelling. However, the primary treatment involves antivenom, which can counteract the effects of the spider’s venom.

Cases of envenomation and fatalities

Although fatalities from Brazilian Wandering Spider bites are uncommon, there have been documented cases of severe envenomation, especially in regions where medical treatment is not readily available. These spiders are generally non-aggressive and will bite humans only in self-defense when provoked, or if they feel cornered.

Understanding the behavior, ecology, and venomous nature of the Brazilian Wandering Spider is crucial for both scientific research and public awareness, helping to minimize the risk of envenomation and promote coexistence with this remarkable but potentially dangerous arachnid.

Brazilian wandering spider life cycle

The Brazilian wandering spider has a fascinating life cycle that revolves around hunting and motherhood. Here’s a breakdown:

• Egg Haven:  After mating, the female lays hundreds, sometimes even a thousand, eggs in a silken sac. This becomes their protected nursery.
• Tiny Terrors:  Hatching from the eggs emerge translucent spiderlings called larvae.
• Nymph Stage:  The larvae molt a few times, transforming into nymphs. Think of them as mini-adults but without the ability to reproduce yet.
• Shedding for Size:  As nymphs grow, they undergo multiple molts, shedding their outer shell to accommodate their larger bodies.
• Ready to Roam:  After the final molt, the spider emerges as a full-fledged adult, complete with reproductive organs. Now, they can join the wandering lifestyle and continue the cycle.

The life cycle begins when a female Brazilian Wandering Spider lays her eggs. She typically creates an egg sac made of silk and deposits it in a concealed location, such as a tree hollow or leaf litter. Inside the sac, she may lay hundreds of eggs. The female guards the egg sac and ensures its protection until the spiderlings hatch. The duration of the egg stage varies depending on environmental conditions but generally lasts for a few weeks.

Spiderling Stage

After the incubation period, spiderlings (young spiders) emerge from the egg sac. They are extremely vulnerable at this stage and rely on their mother’s protection and guidance. Spiderlings are miniature versions of adult spiders but lack the full coloration and size. They disperse from the nest once they have molted and are capable of hunting on their own. During this stage, they grow rapidly by molting, shedding their exoskeletons to accommodate their increasing size.

Juvenile Stage

As spiderlings continue to molt and grow, they progress into the juvenile stage. During this phase, their coloration becomes more distinct, and they start to develop the characteristic features of adult Brazilian Wandering Spiders. They become increasingly independent and begin to exhibit hunting behaviors. The duration of the juvenile stage can vary but often lasts several months.

Sub-Adult Stage

The sub-adult stage is an intermediate phase between juvenile and adult. At this point, the spiders are closer in size and appearance to adults but have not yet reached sexual maturity. They continue to molt, with the frequency of molting gradually decreasing as they approach adulthood. Sub-adult spiders may exhibit more territorial behaviors as they compete for resources and prepare for eventual mating.

Adult Stage

Upon reaching sexual maturity, Brazilian Wandering Spiders enter the adult stage. This is when they are fully developed and capable of reproduction. Females are larger and bulkier than males, and males possess specialized structures called pedipalps, which they use during mating. Adult spiders engage in mating activities, and females lay eggs to continue the life cycle. Adult Brazilian Wandering Spiders can live for several years, with females typically having longer lifespans than males.

Understanding the life cycle of these spiders is essential for studying their behavior, reproductive biology, and population dynamics. It also provides insights into their adaptation strategies in the complex ecosystems of South and Central America.

Brazilian Wandering Spider Bite

Brazilian Wandering Spiders are known for their potent venom and, occasionally, their bites on humans. While bites are relatively rare due to the spider’s non-aggressive nature, understanding the consequences of a bite is crucial for public safety.

Studying Brazilian Wandering Spider bites is of great interest for several reasons. It helps healthcare professionals provide appropriate medical treatment, raises awareness among communities in spider habitats, and contributes to our understanding of venomous arachnid envenomations.

The venom of Brazilian Wandering Spiders is a complex mixture of neurotoxins, cytotoxins, and enzymes. It contains various components, including PhTx3, which is a potent neurotoxin affecting the nervous system.

Brazilian Wandering Spider bites can have a range of effects on the human body, including intense pain, muscle cramps, fever, nausea, and in severe cases, paralysis. The severity of the symptoms depends on factors such as the amount of venom injected and the individual’s sensitivity to the venom. There are several types of spider bites .

Symptoms of a Brazilian Wandering Spider Bite

Local symptoms

• Intense pain
• Itching or burning sensation
• Formation of blisters or lesions

Systemic symptoms

• Muscle cramps and spasms
• Abdominal pain
• Nausea and vomiting
• Elevated heart rate
• Hypertension (high blood pressure)
• Respiratory distress

Severity and variations

The severity of Brazilian Wandering Spider bite symptoms can vary widely. In some cases, symptoms may be mild and resolve on their own, while in severe envenomations, life-threatening complications can occur. Individual reactions to the venom can also vary, making it challenging to predict the exact outcome of a bite.

First Aid and Immediate Response

Steps to take after a bite

Immediate response to a Brazilian Wandering Spider bite should include:

• – Washing the bite area with soap and water.
• – Applying a clean, cool compress to reduce pain and swelling.
• – Immobilizing the affected limb or area.
• – Keeping the bite victim calm to prevent an elevated heart rate.

Do’s and don’ts in case of a bite

• – Seek medical attention promptly.
• – Take note of the spider’s appearance (if possible) to aid identification.
• – Keep the bite victim still and calm to reduce the spread of venom.
• – Do not try to suck out venom or make incisions at the bite site.
• – Avoid applying tourniquets.
• – Don’t use ice directly on the skin as it can worsen tissue damage.

Seeking medical attention

Medical attention is essential after a Brazilian Wandering Spider bite, even if symptoms appear mild initially. Antivenom is available and can be administered to counteract the effects of the venom. Medical professionals can also manage symptoms and monitor for potential complications.

Complications and Long-Term Effects

Potential complications

Complications from Brazilian Wandering Spider bites can include:

• – Severe muscle spasms
• – Respiratory distress
• – Cardiovascular issues
• – Kidney failure (rare)
• – Allergic reactions to antivenom

Long-term consequences

Long-term consequences of a bite can vary depending on the severity and medical treatment received. Some individuals may experience lingering pain, muscle weakness, or psychological trauma following a severe envenomation.

Recovery and rehabilitation

Recovery from a Brazilian Wandering Spider bite typically involves medical treatment, rehabilitation for muscle and nerve damage, and psychological support for individuals affected by the experience. Rehabilitation may include physical therapy to regain muscle strength and function. Prompt medical attention and appropriate care are crucial for minimizing long-term effects and complications.

Facts of Brazilian Wanding Spider

Here are 10 creepy crawly facts about the Brazilian Wandering Spider:

• Big and Hairy:  These spiders are giants! Their bodies can grow up to 2 inches (5 centimeters) long, with a leg span of up to 7 inches (18 cm). They are covered in hairs, making them appear even bigger.
• Wanderlust:  They are aptly named – Brazilian wandering spiders don’t spin webs. Instead, they wander the forest floor at night in search of prey.
• Venomous Bite:  Their venom is considered one of the most potent among spiders. A bite can cause severe pain, swelling, sweating, nausea, and even priapism in males. Thankfully, bites are rare as they are typically shy and defensive.
• Not So Fond of Bananas:  While sometimes called ‘banana spiders,’ they are not typically found in bananas. They might, however, take shelter in them during transport, which is how they might end up in a faraway land.
• Rainforest Resident:  These spiders are native to the rainforests of South America, particularly in Brazil.
• Fearsome Feasters:  They’re active hunters and will eat almost anything they can overpower, including insects, lizards, frogs, and even small rodents.
• Mom’s the Word:  Female Brazilian wandering spiders are dedicated mothers. They lay hundreds of eggs in a silken sac and fiercely guard them until they hatch.
• Multiple Molts:  Like all spiders, Brazilian wandering spiders grow through molting. They shed their exoskeleton multiple times as they grow into adults.
• Mating Dance:  Male Brazilian wandering spiders perform a elaborate mating dance to attract a female.
• Medicinal Potential:  Despite their scary reputation, the venom of the Brazilian wandering spider is being studied for its potential use in treating erectile dysfunction.

What happens if a Brazilian Wandering Spider bites a man?

A Brazilian Wandering Spider bite on a man can cause a range of symptoms, some severe:

• Pain:  The bite is known for causing intense, localized pain at the site.
• Autonomic effects:  Sweating, nausea, and increased heart rate are common.
• Priapism:  In some cases, males may experience a prolonged and painful erection (priapism). This is due to a component in the venom.
• Muscle paralysis:  Severe bites can lead to muscle paralysis, which can affect breathing in rare cases.

However, it’s important to remember:

• Bites are uncommon as these spiders are typically shy and defensive.
• Antivenom is available and effective in treating bites.

If bitten, seek medical attention immediately. Early treatment can help prevent complications and ensure a full recovery.

How poisonous is a Brazilian Wandering Spider?

Brazilian Wandering Spider packs a nasty venomous punch, considered one of the strongest among spiders. Bites are rare though, as they’re shy and prefer to escape trouble.

What is the world’s deadliest spider?

The term “deadliest” can be subjective and depends on how one defines it (e.g., based on venom potency or human fatalities). However, the Brazilian Wandering Spider (Phoneutria) is often considered one of the most venomous spiders in the world. Another spider often mentioned in discussions of venom toxicity is the Sydney Funnel-web Spider (Atrax robustus and Atrax formosus), found in Australia. These spiders are known for their potent venom and have caused fatalities in the past.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Save my name, email, and website in this browser for the next time I comment.

Related Article

Can a Daddy Long Legs Bite Kill You (You Need to Know)

7 Facts About The Brazilian Wandering Spider

Are Wolf Spiders Poisonous for Humans, Cats or Dogs? (Explained)

When To Worry About A Spider Bite? (Need to Know)

AnimalBehaviorCorner

Brazilian Wandering Spider

Brazilian Wandering Spider , scientifically known as Phoneutria, emerges as a captivating enigma in the realm of arachnids.

Renowned for its formidable reputation as one of the world’s most venomous spiders , Phoneutria embodies a plethora of intriguing traits that have captured the curiosity of enthusiasts and researchers alike.

From its distinctive appearance and neurotoxic venom to its nomadic hunting strategies and unique mating behaviors , this remarkable spider species holds a wealth of fascinating secrets waiting to be unraveled.

Join us as we embark on a journey to explore the captivating world of the Brazilian Wandering Spider, shedding light on its captivating characteristics and dispelling myths that have shrouded its true nature.

1. Taxonomy and Distribution of the Brazilian Wandering Spider

A. scientific classification of phoneutria.

The Brazilian Wandering Spider, scientifically referred to as Phoneutria, occupies a distinct place within the arachnid taxonomy.

Belonging to the family Ctenidae, this spider genus is further categorized into several species, each boasting unique traits and behaviors .

Phoneutria’s taxonomic position not only distinguishes it from its arachnid counterparts but also underscores its intriguing evolutionary journey.

B. Native Habitat in South and Central America

Endemic to the lush landscapes of South and Central America, the Brazilian Wandering Spider finds its natural haven within these diverse regions.

From the rainforests of the Amazon to the tropical stretches of the Caribbean, Phoneutria has adapted to a range of environments over the course of its evolution.

The spider’s ancestral ties to these regions are tightly woven into their behaviors , anatomy, and survival strategies.

C. Preference for Tropical Rainforests and Urban Areas

Within its native territories, the Brazilian Wandering Spider exhibits remarkable versatility in its chosen habitats.

While it thrives amidst the vibrant biodiversity of tropical rainforests, it has also displayed a propensity for urban locales.

Phoneutria’s adaptability has led it to establish a presence in urban areas, where it often finds shelter in crevices, gardens, and even human dwellings.

This adaptability to both wild and urban spaces further showcases the spider’s resilience and capacity to thrive in varying conditions.

2. Physical Characteristics of the Brazilian Wandering Spider

A. size, coloration, and distinctive markings.

The Brazilian Wandering Spider , a creature of remarkable visual intrigue, boasts an array of captivating physical attributes.

Ranging in size from a few centimeters to several inches, Phoneutria showcases a size diversity that reflects the breadth of its genus.

Its coloration varies across species, encompassing shades of brown, black, and gray, often accompanied by intricate patterns and markings that adorn its exoskeleton.

These unique markings serve not only as a visual spectacle but also as essential components of its survival toolkit.

B. Camouflage and Defense Mechanisms

The Brazilian Wandering Spider’s appearance is a masterpiece of evolution, meticulously crafted to ensure both survival and predation .

Its coloration and markings are tailor-made for blending seamlessly into its surroundings, granting it a potent advantage in ambushing prey and evading predators . Moreover, these markings also play a role in its defense mechanisms.

When threatened, Phoneutria adopts a defensive posture, raising its front legs and revealing its striking markings, a visual warning to potential threats. This dual-purpose camouflage and defense strategy exemplify nature’s ingenuity at its finest.

C. Sexual Dimorphism: Unveiling Gender Differences

A fascinating facet of the Brazilian Wandering Spider lies in the realm of sexual dimorphism , where gender-based variations manifest in pronounced ways.

Females tend to be larger and more robust than their male counterparts, showcasing a size disparity that has evolved in tandem with their roles in reproduction and hunting .

Beyond size, other characteristics, such as leg structure and coloration, also exhibit subtle differences between male and female Phoneutria specimens.

This divergence in physical traits adds depth to our understanding of the species’ intricate biology and behavior .

In exploring the physical characteristics of the Brazilian Wandering Spider , we uncover a canvas painted with size diversity, intricate coloration, and unique markings.

These features, finely tuned by evolution, contribute to its prowess in camouflage and defense, while the fascinating interplay of sexual dimorphism further enriches our perception of this captivating arachnid species .

3. Venomous Nature of the Brazilian Wandering Spider

A. potent neurotoxic venom: a silent lethal weapon.

The Brazilian Wandering Spider, known scientifically as Phoneutria, harbors a venomous arsenal that stands as a testament to nature’s intricate design.

This spider’s venom contains a potent concoction of neurotoxic compounds, tailored by evolution to incapacitate its prey swiftly and efficiently.

The neurotoxins interfere with nerve cell communication, leading to paralysis and ensuring that Phoneutria’s quarry is rendered immobile and defenseless, setting the stage for a successful meal.

B. Effects on Prey and Human Hazard

When a victim succumbs to the Brazilian Wandering Spider’s venom , the effects are a symphony of paralysis and predation .

The venom’s impact on the prey’s nervous system results in swift immobilization, offering the spider a decisive advantage in subduing its catch.

While this venomous efficiency is well-adapted for predation, it also underscores the potential danger to humans.

A bite from Phoneutria can lead to a series of neurotoxic reactions, with varying degrees of severity depending on factors such as the individual’s age and overall health.

While human envenomations are relatively rare, they can result in a range of symptoms, from localized pain and swelling to more severe neurological effects.

C. Recorded Cases of Envenomations: Unraveling the Symptoms

Throughout history, documented cases of Phoneutria envenomations have offered insights into the spider’s potential threat to humans .

Symptoms typically include intense pain at the bite site, accompanied by swelling and redness . In some instances, victims have reported systemic reactions, such as muscle cramps, elevated heart rate, and even breathing difficulties.

Swift medical attention and the administration of antivenom have proven effective in mitigating the severity of these symptoms.

These cases serve as a reminder of the delicate balance between the Brazilian Wandering Spider’s potent venom and the potential risks it poses to those who unwittingly encounter it.

4. Hunting and Diet of the Brazilian Wandering Spider

A. hunting techniques and wandering behavior.

The Brazilian Wandering Spider, scientifically known as Phoneutria, unveils a mesmerizing repertoire of hunting techniques that set it apart as a master predator .

Displaying an agile and nomadic behavior , Phoneutria does not confine itself to the confines of a web. Instead, it actively prowls its surroundings, tirelessly searching for potential prey.

This dynamic wandering behavior ensures that its chances of encountering a variety of food sources are maximized, showcasing a strategic approach to sustenance.

B. Active Hunting Triumphs Over Web-Building

Unlike its web-weaving counterparts, the Brazilian Wandering Spider relies on a more hands-on approach to securing its next meal.

While weaving webs might seem an efficient method, Phoneutria’s active hunting strategy offers a distinct advantage in versatility.

By forgoing the constraints of a stationary web, it can tailor its approach to suit different environments and prey types, adapting its tactics on the fly.

This adaptability demonstrates the spider’s remarkable ability to adjust its methods for optimal results.

C. Diverse Prey Spectrum: Insects to Small Vertebrates

Phoneutria’s diet is a testament to its prowess as an opportunistic predator . Its menu spans a diverse range of creatures, from insects like crickets and cockroaches to small vertebrates such as lizards and frogs , and even small rodents.

This wide-ranging palate highlights its ecological significance in controlling various populations within its habitat.

By consuming creatures both large and small, Phoneutria ensures a balanced ecosystem, playing a crucial role in maintaining biodiversity and ecological equilibrium.

5. Mating and Reproduction of the Brazilian Wandering Spider

A. courtship rituals and behaviors: a complex affair.

The Brazilian Wandering Spider, scientifically referred to as Phoneutria, reveals a captivating array of courtship rituals and behaviors that form the cornerstone of its reproductive cycle.

Courtship among these arachnids is a complex affair, involving intricate dances and displays that serve as both communication and assessment.

Male Phoneutria employs a combination of visual cues, vibrations, and tactile interactions to court potential mates.

This elaborate courtship process highlights the significance of precise communication in the delicate dance of reproduction .

B. Cannibalistic Tendencies: A Post-Mating Phenomenon

An aspect that sets Phoneutria’s mating process apart is the notorious cannibalistic tendency exhibited by females after mating.

Following successful mating, females may exhibit an inclination to consume their partners. This seemingly counterintuitive behavior has evolutionary underpinnings.

It is believed that this cannibalistic act not only provides the female with a much-needed nutritional boost but also eliminates potential competitors and safeguards the male’s investment in the next generation.

This intriguing behavior sheds light on the complexities of reproductive strategies within the species.

C. The Unique Mating Plug Phenomenon: A Puzzling Enigma

A distinctive feature in Phoneutria’s reproductive saga is the enigmatic mating plug phenomenon. After mating, male Phoneutria deposit a specialized substance that forms a plug within the female’s reproductive tract.

This plug is believed to serve multiple purposes. It may prevent other males from mating with the female, thus ensuring the successful transmission of the mating male’s genetic material.

Additionally, it might aid in sealing off the female’s reproductive tract, potentially protecting her from external pathogens.

This phenomenon underscores the intricate interplay of biological strategies that contribute to the species’ reproductive success.

6. Human Interaction and Urban Legends of the Brazilian Wandering Spider

A. occasional presence in urban areas: nature in our midst.

The Brazilian Wandering Spider, scientifically known as Phoneutria, has carved a niche for itself not only in the wild but also in the fabric of urban environments.

While its primary habitats are the lush landscapes of South and Central America, Phoneutria occasionally ventures into human -inhabited spaces. Its adaptability allows it to find shelter in gardens, crevices, and even within homes.

This coexistence with humans adds an intriguing dimension to our encounters with this enigmatic arachnid .

B. Debunking Misconceptions: Separating Fact from Fiction

The presence of the Brazilian Wandering Spider has sparked a plethora of misconceptions and exaggerated tales, contributing to the creation of urban legends.

Stories of spiders leaping from banana bunches or hiding under toilet seats have become part of modern folklore, often fueled by sensationalism.

It’s crucial to sift through these tales and recognize that while Phoneutria’s venom is potent, the likelihood of encountering a dangerous encounter is relatively low.

Separating fact from fiction empowers individuals to approach these creatures with accurate knowledge.

C. Importance of Proper Education: Identifying Friend from Foe

Education plays a pivotal role in fostering a harmonious coexistence between humans and the Brazilian Wandering Spider .

Learning to identify and understand the behaviors of Phoneutria species enhances safety for both humans and the spiders themselves.

Instead of succumbing to unwarranted fear, individuals can take steps to reduce the chances of accidental encounters and, if necessary, engage in responsible removal methods.

By arming themselves with knowledge, individuals can navigate encounters with urban-dwelling Phoneutria specimens with confidence and respect.

7. Brazilian Wandering Spider Conservation and Misunderstanding

A. significance of phoneutria in ecosystem dynamics.

The Brazilian Wandering Spider , scientifically termed Phoneutria, assumes a pivotal role within its ecosystem, contributing to a delicate balance of populations and interactions.

As a top-tier predator , it plays a crucial part in controlling insect and small vertebrate populations, preventing unchecked growth that could disrupt the ecosystem’s equilibrium.

By maintaining these population dynamics, Phoneutria ensures the health and stability of its habitat, highlighting its significance beyond its ominous reputation.

B. Impact of Fear and Misunderstanding: Hindrances to Conservation

Despite its ecological contributions, the Brazilian Wandering Spider often falls victim to fear-driven misconceptions that negatively impact conservation efforts.

Misunderstandings surrounding its behavior and potential danger can lead to unwarranted extermination campaigns and habitat destruction.

Fear-driven reactions not only disrupt the natural balance but also hinder opportunities to study and appreciate the species for its ecological significance.

Addressing these misconceptions is crucial to ensuring the spider’s survival and maintaining the health of its ecosystems.

C. Efforts to Dispel Myths and Promote Coexistence

Efforts to conserve the Brazilian Wandering Spider are interwoven with endeavors to educate and dispel myths.

By providing accurate information and dispelling exaggerated tales, conservationists aim to reshape public perception.

Collaborative initiatives emphasize coexistence, highlighting the importance of responsible behavior when encountering Phoneutria.

Educating communities about the spider’s role, behavior, and conservation status fosters an environment where fear gives way to appreciation, and where balanced cohabitation becomes a reality.

8. Research and Medical Significance of the Brazilian Wandering Spider

A. ongoing scientific research on phoneutria venom.

The Brazilian Wandering Spider , Phoneutria, has garnered significant attention from the scientific community due to the unique properties of its venom.

Ongoing research delves into the intricate composition of the venom, aiming to unlock its mysteries and potential applications in various fields.

The diverse array of compounds within the venom, particularly its neurotoxic components, has attracted interest for their potential medical and therapeutic implications.

B. Antivenom Development and Therapeutic Prospects

One of the most promising areas of research surrounding Phoneutria lies in the development of antivenoms and therapeutic agents.

The venom’s potent neurotoxic effects on the nervous system have spurred efforts to create targeted treatments for conditions such as chronic pain and neurological disorders .

Additionally, the potential for antivenoms holds promise in mitigating the effects of envenomations, offering a lifeline for individuals who encounter these spiders .

This focus on harnessing the venom’s properties for positive medical outcomes highlights the transformative potential within this enigmatic arachnid .

C. Balanced Perspectives: Navigating Ethical and Scientific Endeavors

While research on the Brazilian Wandering Spider’s venom offers tremendous potential, it necessitates a balanced perspective.

As researchers probe the venom’s properties, ethical considerations arise, including the well-being of the spiders and their ecosystems.

A holistic approach acknowledges the value of understanding Phoneutria’s natural behaviors and conserving its habitats.

This balanced perspective extends to utilizing the venom’s potential responsibly, ensuring that breakthroughs are achieved while respecting the complex interplay of science and nature.

9. Frequently Asked Questions about the Brazilian Wandering Spider

What is the brazilian wandering spider.

The Brazilian Wandering Spider , scientifically known as Phoneutria, is a venomous arachnid found in South and Central America. It’s notorious for its potent venom and is considered one of the most venomous spiders in the world.

Is the Brazilian Wandering Spider dangerous to humans?

Yes, the Brazilian Wandering Spider’s venom contains potent neurotoxins that can cause a range of symptoms in humans , from localized pain and swelling to more severe reactions. While bites are relatively rare, it’s advisable to exercise caution when encountering these spiders.

What is the spider’s habitat?

The Brazilian Wandering Spider is native to tropical rainforests of South and Central America. However, it’s adaptable and can also be found in urban areas, such as gardens and houses.

How does the Brazilian Wandering Spider hunt?

Unlike many spiders that build webs, Phoneutria is an active hunter. It roams its environment in search of prey, relying on its keen senses to detect vibrations and movements.

Are Brazilian Wandering Spiders aggressive toward humans?

Brazilian Wandering Spiders are not naturally aggressive towards humans and will typically only bite in self-defense. However, caution is advised, especially in areas where these spiders are known to inhabit.

Can the Brazilian Wandering Spider’s venom be used for medical purposes?

Yes, research is ongoing into the potential medical applications of Phoneutria’s venom. Its neurotoxic properties have sparked interest in pain management and neurological treatments.

Is the spider’s reputation for crawling into banana shipments true?

While there have been stories of Brazilian Wandering Spiders being found in shipments of bananas, these occurrences are extremely rare. Spiders are unlikely to survive the conditions of shipping and storage.

How can I stay safe around Brazilian Wandering Spiders?

To stay safe, it’s important to be cautious when encountering spiders in their natural habitat. Avoid provoking or handling them, especially if you’re unsure of their identity. If you suspect you’ve been bitten, seek medical attention promptly.

Are there any efforts to conserve the Brazilian Wandering Spider?

Conservation efforts for the Brazilian Wandering Spider are intertwined with public education and dispelling myths. Recognizing its role in ecosystems and promoting coexistence are essential steps in preserving this unique species.

What can I do if I find a Brazilian Wandering Spider in my home?

If you encounter a Brazilian Wandering Spider in your home, it’s advisable to contact local pest control professionals who can safely remove the spider without causing harm.

In the intricate tapestry of nature, the Brazilian Wandering Spider , Phoneutria, emerges as a creature of both fascination and caution.

Its venomous nature and captivating behaviors have earned it a place among the most enigmatic arachnids .

As we continue to explore its world, debunk myths, and understand its vital role in ecosystems, we find a delicate balance between awe and respect.

Armed with knowledge, we navigate the realm of Phoneutria, appreciating its complexity while fostering coexistence, a testament to the intricate dance between humans and the natural world.

Share this:

Similar posts.

Lobster Behaviour

Lobster behaviour is a fascinating and intricate aspect of marine biology that has intrigued scientists and enthusiasts alike. These crustaceans, known for their distinctive appearance and delectable taste, exhibit a range of behaviors that are not only essential for their survival but also offer a captivating glimpse into the natural world beneath the waves. Understanding…

Tasmanian Devil Behavior

Tasmanian devils are fascinating creatures that are native to the island state of Tasmania, Australia. These carnivorous marsupials are known for their unique behavior and have captured the attention of scientists and animal lovers alike. Understanding Tasmanian devil behavior is important for conservation efforts as their populations have been impacted by disease and human activity….

What Are Animal Behaviors?

Animal behaviors are the studies of animal actions and reactions to stimuli. It can be divided into two categories: instinctive behavior and learned behavior. Instinctive behavior is carried out automatically, without any conscious thought on the part of the animal. Learned behavior, on the other hand, is something that an animal learns after being exposed…

Lionfish Behavior

Lionfish Behavior is a fascinating subject that sheds light on the intriguing characteristics and habits of these mesmerizing marine creatures. Lionfish, known for their striking appearance and invasive presence in certain regions, exhibit a range of behaviors that contribute to their survival and impact on local ecosystems. In this article, we will delve into the…

Animal Behavior Research

Animal Behavior Research unveils the captivating mysteries of the animal kingdom, delving into the intricacies of how creatures behave, communicate, and interact within their environments. This fascinating field of study goes beyond mere observation, employing advanced methodologies and cutting-edge technologies to decipher the language of the wild. As we embark on a journey through the…

Starfish Behavior

Starfish behavior is a fascinating subject that delves into the intricate world of marine life. These captivating creatures, also known as sea stars, exhibit a myriad of behaviors that unveil their unique adaptations and survival strategies in diverse aquatic ecosystems. From their remarkable ability to regenerate lost limbs to their methodical hunting techniques, starfish embody…

JUST SPIDERS

Brazilian wandering spider phoneutria spp.  is actually a genus with five known similar species whose members are highly venomous. they include some of the relatively few species of spiders that present a threat to human beings. the brazilian wandering spider (phoneutria fera) can grow to have a leg span of up to 4-5 inches..

These spiders are notorious both due to their toxic venom, and because they are not reluctant to attack people who appear threatening.

Of the five species known, P. nigriventer and P. fera most frequently receive mention in mass-media publications. P.nigriventer species are responsible for most cases of venom intoxication in Brazil because this species is commonly found in highly populated areas of Brazil, namely the South-eastern states: São Paulo, Minas Gerais, Rio de Janeiro and Espírito Santo.

The P. fera is native to the northern portion of South America, especially the Brazilian Amazônia, Venezuela and French Guiana.

Photo right: P. nigriventer female (Sao Paolo, Brazil morph). 

Recent studies suggest that these spiders only inject venom in approximately one-third of their bites and may only inject a small amount in another third. However, research in this area is hindered by the difficulty of identifying particular subspecies. Thus the effects of the bites from these spiders are hard to predict when based on sketchy information or if the spider has not been identified definitively.  

Bites from these spiders may result in only a couple of painful pinpricks to full-blown envenomation. In either case, people bitten by a Phoneutria or any Ctenid should seek immediate emergency treatment as the venom is possibly life threatening.

Photo left: Dead P. reidyi

Help keep Spiderzrule going. Click to donate!

The Phoneutria fera and nigriventer  are the two most commonly implicated as the most virulent of the Phoneutria spiders. The Phoneutria not only has a potent neurotoxin, but is reported to have one of the most excruciatingly painful envenomations of all spiders due to its high concentration of serotonin.  

Brazilian Wandering Spiders are extremely fast, extremely venomous, and extremely aggressive and are ranked among the most venomous spiders known to man. In fact, the Brazilian Wandering Spider is the most venomous spider in the New World! In South America, these true spiders are commonly encountered in peoples' homes, supposedly hiding in peoples' shoes, hats, and other clothes. It does not remain on a web, rather, it wanders the forest floor, which is how it got its name.

The Brazilian Wandering Spider has another name - the Banana Spider and it was given this name  because there have been cases where these spiders unintentionally appeared on banana boats heading for the United States.

Click here for more photos of Wandering Spiders.

Information and pictures were taken from children's projects and where credited to that child does not claim to be original information. Where possible, permission to reproduce has been sought. Any infringement of copyright is purely unintentional.

Understanding the Wandering Spider: Quick Essential Facts

Wandering spiders are a group of venomous arachnids found primarily in South America.

Among these, the Brazilian wandering spider is particularly known for its potent venom and unique behavior. They are often referred to as “banana spiders” due to their frequent encounters with humans in banana plantations.

As a reader, you might be interested in learning more about these fascinating creatures, including their habitat, hunting techniques, and the effects of their venom.

In this article, we will delve into the world of wandering spiders and provide you with all the essential information to satisfy your curiosity.

Scientific Classification and Naming

The wandering spider belongs to the genus Phoneutria , which is a part of the Ctenidae family.

These spiders are known for their potent venom and aggressive behavior. Here is the scientific classification of the wandering spider:

• Kingdom: Animalia
• Phylum: Arthropoda
• Subphylum: Chelicerata
• Class: Arachnida
• Order: Araneae
• Family: Ctenidae
• Genus: Phoneutria

Within the genus Phoneutria, two species are particularly noteworthy: Phoneutria fera and Phoneutria nigriventer, also known as P. nigriventer . These spiders are primarily found in South America and other tropical regions.

Phoneutria fera and P. nigriventer differ in some aspects. Let’s compare their features using a table:

Some key characteristics of the wandering spiders in the genus Phoneutria include:

• Potent venom that can be dangerous to humans
• Nocturnal hunters and are active at night
• Equipped with long, spiny legs for capturing prey
• Aggressive defenders of their territory

By understanding the scientific classification and differences between Phoneutria species, you can better appreciate the diversity and fascinating biology of these wandering spiders.

Identification and Appearance

Color and size.

The wandering spider, also known as the banana spider, has a distinctive appearance that can help you easily identify it in the wild.

They usually have a combination of hairy brown and black colors on their body. Their size can vary, but they are generally considered large spiders. Their size can range from 1 to 2 inches in body length.

When it comes to wandering spider’s leg span, these creatures can have an impressive reach. Their leg span can extend up to 5-6 inches.

Some key characteristics of a wandering spider’s legs include:

Habitat and Distribution

Wandering Spiders are known to inhabit various environments, including rainforests and tropical forests.

These spiders can adapt to different habitats based on their needs and availability of food sources. They prefer warm and humid places, as these conditions suit their growth and reproduction.

Geographical Coverage

Wandering spiders are found in Central and South America .

They live in forests from Costa Rica to Argentina, including Colombia, Venezuela, The Guianas, Ecuador, Peru, Bolivia, Brazil, Paraguay, and Northern Argentina.

They may also be present in some parts of the United States, particularly in the northern part of southern America.

However, they don’t inhabit countries like Australia. In summary, the Wandering Spider is mostly prevalent in the following areas:

• South America
• Central America
• Southern parts of the United States

Types of Wandering Spiders

Here’s a brief description of the major types of wandering spiders.

Brazilian wandering spiders

Also known as armed or banana spiders, these spiders are nocturnal and don’t make webs.

They are known to have been transported outside of South America in banana shipments.

Phoneutria nigriventer

These spiders contain neurotoxins that can cause cerebral changes and breakdown of the blood-brain barrier .

Their venom is medically significant and has been used in manufacturing drugs. Their bites may be fatal to children.

Ctenus captiosus

Also known as the Florida false wolf spider or tropical wolf spider, this species is found in the United States.

Some species of these spiders are large and scary-looking, but they’re only mildly venomous. Their venom is comparable to a bee sting.

Other types of wandering spiders include: Acantheis, Acanthoctenus, Africactenus, and Afroneutria.

Behavior and Diet

Aggression level.

Wandering spiders, as their name suggests, are known for their aggressive behavior .

While they won’t attack without provocation, if they feel threatened, they will not hesitate to defend themselves.

This is especially true during mating season.

Prey and Predators

In their natural habitat, wandering spiders primarily feed on insects and small vertebrates, such as:

• Insects like ants and moths
• Small amphibians

This diverse diet allows them to thrive in various ecosystems.

However, they are not top predators, as their natural predators include larger birds, mammals, and other spiders.

Nocturnal Activities

Wandering spiders are nocturnal creatures , which means they are active during the night.

During the day, they remain hidden in their retreats, often made from rolled-up leaves or small crevices.

At night, they leave their hiding spots to search for prey using their strong hunting skills.

Venom and Its Effects

Composition of venom.

The venom of the wandering spider is a complex mixture containing several toxic components.

Its main component is a potent neurotoxin, which can have severe effects on your nervous system 1 . Here’s a brief overview of its composition:

• Neurotoxins

Symptoms and Severity

A wandering spider’s venomous bite can cause a wide range of symptoms, depending on the severity of envenomation. These symptoms may include 2 :

• Mild to moderate pain
• Redness and swelling at the bite site
• Irregular heartbeat
• Difficulty breathing
• Blurred vision
• High blood pressure

Some severe cases may result in life-threatening complications, such as respiratory failure or even death 2 .

Medical Treatment and Antivenom

If bitten by a wandering spider, it’s crucial to seek immediate medical attention. Treatment often involves the following steps:

• Cleaning and immobilizing the affected area
• Monitoring and managing the symptoms
• Administering antivenom if it’s available and appropriate, depending on the severity of envenomation 3

Antivenom is specific to the venom of the wandering spider and can help neutralize its effects.

However, the availability of antivenom may be limited in some regions 3 .

Always remember that prevention is better than cure: learning how to identify and avoid wandering spiders is the best way to stay safe.

Reproduction and Mating

Mating ritual.

When it’s time for reproduction, the wandering spider undergoes an intriguing mating ritual.

The male spider performs a dance to attract the female by displaying his brightly colored legs and vibrating his body.

During the process, the male also produces a sperm web and transfers his sperm to the female’s reproductive organs using his pedipalps.

Egg Sacs and Offspring

After the mating process, the female wandering spider will create an egg sac to protect her eggs.

The sac consists of silk and can hold hundreds of eggs. She then attaches it to a safe hiding place, usually against a protective surface or within a secure web.

The female often guards the egg sac to ensure the protection of her offspring until they hatch.

Once the spiderlings hatch, they are known to be highly independent.

They disperse quickly and start their own journey, fending for themselves soon after emerging from the egg sac.

As they grow, they’ll go through a series of molts before reaching adulthood and beginning their own reproductive cycle.

Danger and Defense Mechanisms

The Wandering Spider is known to be one of the most dangerous spiders in the world.

Although they can potentially kill humans, fatalities are rare due to their reluctance to bite.

Oddly enough, their venom can cause an involuntary erection in men, alongside other painful symptoms.

Here are some ways the Wandering Spider protects itself and displays its dangerous nature:

• Fangs : These spiders are equipped with strong, sharp fangs that can easily pierce human skin, allowing them to inject their venom with ease.
• Venom : Their venom is potent and can cause severe pain, inflammation, and other adverse effects. In rare cases, it can even lead to death.

While interacting with Wandering Spiders, be cautious and observe them from a safe distance.

Knowing their defense mechanisms will help you respect their space and avoid any unpleasant encounters.

Remember, it’s essential to be informed and aware when dealing with these fascinating, yet dangerous creatures.

Comparison with Other Dangerous Spiders

Comparison to black widow.

The black widow spider is notorious for its potent venom, but the wandering spider has a stronger venom overall.

Both spiders are capable of causing severe symptoms, but the black widow’s venom is primarily neurotoxic, affecting your nervous system.

In contrast, the wandering spider’s venom can cause both neurotoxic and cytotoxic effects, potentially damaging your nerves and cells.

• Potent neurotoxic venom
• Red hourglass marking
• Stronger venom (neurotoxic and cytotoxic)
• No distinct marking

Comparison to Brown Recluse

The brown recluse spider is known for its necrotic venom that can lead to tissue damage and sometimes requires medical intervention.

While both the brown recluse and wandering spider can produce venomous bites, wandering spiders are considered more dangerous due to the potency of their venom and the severity of their bite symptoms.

• Necrotic venom
• Dark violin-shaped marking

Comparison to Wolf Spider

Wolf spiders are frequently mistaken for more dangerous spiders due to their size and appearance.

Although they can bite, their venom is not particularly potent and generally only causes mild itching, redness, and swelling.

In comparison, the wandering spider’s venom is far more dangerous, and its bite can result in serious symptoms, requiring immediate medical attention.

• Large and hairy
• Smoother appearance

Comparison to Sydney Funnel-Web Spider

The Sydney funnel-web spider is another highly venomous spider known for its potentially lethal bites.

While both spiders possess powerful venom, the wandering spider has a broader range of symptoms due to the combination of neurotoxic and cytotoxic effects.

In conclusion, wandering spiders are more dangerous than wolf spiders but their venom’s effects are more varied compared to black widows, brown recluses, and Sydney funnel-web spiders.

Be cautious around these spiders and seek medical help if bitten.

Interesting Facts and Guinness World Records

The Wandering Spider, also known as the Brazilian Wandering Spider, is a fascinating creature that has caught the attention of many.

They belong to the genus Phoneutria , which means “murderess” in Greek, giving you an idea of their potency. Let’s explore some interesting facts about this spider and its place in the Guinness World Records.

First, you might be curious about their venom. The Wandering Spider is known for having one of the most potent venoms among spiders.

In fact, it holds the Guinness World Record for the most venomous spider. Their venom contains a potent neurotoxin that can cause severe symptoms, including difficulty breathing, high blood pressure, and intense pain.

Apart from their venom, their behavior is also quite intriguing. These spiders are called “wandering” because they are known for actively hunting their prey rather than spinning webs to catch them.

They are mostly nocturnal creatures and, during the day, can be found hiding in logs or dark crevices.

Here are a few more notable characteristics of the Wandering Spider:

• Females are larger than males, with a body length of up to 1.6 inches (4 cm).
• They have eight eyes, arranged in two rows, which help them in hunting.
• The Wandering Spider is primarily found in Central and South America, particularly in Brazil.
• They are known to show aggression when threatened.

While the Wandering Spider is a marvel of the arachnid world, it’s essential to keep a safe distance from them due to their venomous nature.

However, their unique characteristics and record-breaking venom potency make them a fascinating subject for those interested in the natural world.

Prevention and Safety Measures

To protect yourself from wandering spiders, there are some simple safety measures you can take.

Firstly, be cautious in areas where these spiders may live, such as dark and warm spaces. For example, avoid reaching into crevices or lifting piles of wood without inspecting them first.

Always wear appropriate shoes when outdoors, particularly in wooded or grassy areas. This can help prevent bites on your feet or ankles.

Reduce the risk of wandering spiders entering your home by sealing gaps and cracks. This minimizes the chance of the spiders finding a way inside.

Regularly clean your living spaces, paying special attention to dark and hidden areas. By maintaining a clean environment, you’ll discourage wandering spiders from making themselves at home.

When out in nature, avoid disturbing spider habitats like webs or egg sacs. This can prevent agitating wandering spiders, reducing your chance of accidental encounters.

Remember, wandering spiders can be dangerous, but by taking these precautions, you can significantly reduce your risk of encountering them or being bitten. Stay safe and always be aware of your surroundings.

In summary, wandering spiders, particularly those in the genus Phoneutria, are a group of venomous arachnids predominantly found in Central, South America and parts of Southern United States.

These spiders, including the Brazilian wandering spider, are known for their potent venom, nocturnal hunting habits, and aggressive defense mechanisms.

Their venom, containing neurotoxins and other components, can cause severe symptoms in humans, making them one of the most dangerous spider species.

Despite their fearsome reputation, fatalities are rare, and they play a vital role in their ecosystems.

It’s important to respect their space and take preventive measures to avoid encounters. Understanding these spiders’ behavior, habitat, and characteristics can help in appreciating their role in nature while ensuring safety.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857337/ ↩

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851068/ ↩ ↩ 2

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560916/ ↩ ↩ 2

Reader Emails

Over the years, our website, whatsthatbug.com has received hundreds of letters and some interesting images asking us about wandering spiders. Scroll down to have a look at some of them.

Letter 1 – Wandering Spider from Ecuador

Hi Michele, There is a resemblance to the Dolomedes Fishing Spiders, and finding it near a river lends credence to that possibility. Eric Eaton noticed this posting and has this to say: ” Ok, the spiders from Ecuador and Costa Rica: They are most likely NOT wolf spiders, but wandering spiders, either in the family Ctenidae or Sparassidae. They tend to be more common, and even larger than, wolf spiders in the tropics. At least one species, Phoneutria fera, is extremely aggressive, with potentially deadly venom. Do not mess with large spiders in Central and South America! The venomous types are very difficult to distinguish from harmless species, and in any event, a bite is going to be really painful. These spiders sometimes stow away in bananas, houseplants, and other exported goods, so they can show up in odd places. Be careful where you put your hands.”

Update:  May 14, 2013 We now have a confirmation that this is a Wandering Spider, Phoneutria fera , and it is a dangerous species.  See Encyclopedia Britannica and Animal Corner .

Letter 2 – Brazilian Wandering Spider: Most Venomous Animal

Hi Martin, We are happy you were able to write to us after your encounter with this Brazilian Wandering Spider and are thrilled to be able to post your story and photos to our site. We started to research, and our first hit has a different species name. Phoneutria fera is described as: “The Brazilian Wandering Spider is not for the ‘pet keeper’. Brazilian Wandering Spiders are extremely fast, extremely venomous, and extremely aggressive. These large and dangerous true spiders are ranked among the most venomous spiders known to man. In fact, the Brazilian Wandering Spider is the most venomous spider in the New World! In South America, these true spiders are commonly encountered in peoples’ homes, supposedly hiding in peoples’ shoes, hats, and other clothes. The Brazilian Wandering Spider does not remain on a web, rather, it wanders the forest floor, hence the name.” Our favorite information on Wikipedia is that Phoneutria is Greek for “murderess”. Here is one final tidbit about the effect of the bite of the Brazilian Wandering Spider on the human male .

Letter 3 – Possibly Wandering Spider from Ecuador

Dear Mike, This is really an interesting Spider, but other than to say it appears to be a hunting spider that does not build a web to entrap prey, we aren’t sure about its identity.  Many hunting spiders can jump quite well.  It looks very much like the spider in a posting in our archives, also from Ecuador, that we identified as possibly a Wandering Spider in the genus Phoneutria, a venomous and potentially dangerous genus .  The spotted legs on your individual look like the spotted legs on an individual in an image on Wikipedia of a Wandering Spider in the genus Phoneutria .  There are many images of Brazilian Wandering Spiders on Primal Shutter and we believe that might be a correct identification for your individual.

Thank you for the information.  After reading more about the spider, I’m glad it didn’t jump! Mike

Letter 4 – Possibly Wandering Spider from Ecuador

Dear Carl, We believe, though we are not certain, that this might be a Wandering Spider in the genus Phoneutria, and you may read more about Wandering Spiders on the Museum für Naturkunde Karlsruhe website where it states:  “There is no doubt that the venom of some of the species is quite potent for mammals, including humans.”  We eagerly welcome additional opinions on this identification.  Perhaps Cesar Crash of Insetologia can provide something.  In the future, please submit a single species per submission form as it makes it extremely difficult for us to categorize postings with multiple species.

Letter 5 – Wandering Spider from Belize

Hi Karl, Thanks for allowing us to post your excellent image of a Wandering Spider, Cupiennius salei .  The species is pictured on iNaturalist .

Bugman aka Daniel Marlos has been identifying bugs since 1999. whatsthatbug.com is his passion project and it has helped millions of readers identify the bug that has been bugging them for over two decades. You can reach out to him through our Contact Page .

View all posts

Piyushi is a nature lover, blogger and traveler at heart. She lives in beautiful Canada with her family. Piyushi is an animal lover and loves to write about all creatures.

8 thoughts on “Understanding the Wandering Spider: Quick Essential Facts”

Hi Michele, I am an Ecuadorian scientist and specialized on spiders, I would like to find one like yours, I can say that, almost without doubt, you found the Phoneutria itself, it is the Phoneutria fera, look at this picture: http://www.google.com/imgres?imgurl=http://4.bp.blogspot.com/-bFH9qzT0F7U/T_2sZuk6xAI/AAAAAAAAAGY/8jnMVcPOcNI/s1600/phoneutria_fera2.jpg&imgrefurl=http://rangerbaiano.blogspot.com/2012/07/animais-peconhentos-e-venenosos.html&usg=__iCWEz7S86xub6RAyvXTER6HBaco=&h=864&w=834&sz=215&hl=es-419&start=6&zoom=1&tbnid=jjOROVO9h-vKXM:&tbnh=145&tbnw=140&ei=99eRUY6xKo2K9QTLvYCoDQ&prev=/search%3Fq%3Dphoneutria%2Bfera%26sa%3DN%26hl%3Des-US%26sout%3D1%26tbm%3Disch%26prmd%3Divns&itbs=1&sa=X&ved=0CDYQrQMwBQ Can you see the similarities?, unfortunately the spider might be in a better life today 🙂 Another thing, when you want identifications you should take a picture in front, the under part, and the upper part, as well as some characteristics about behaviour like how they react when you approach. The Phoneutria is a very agressive one.. best wishes, bye.

Hi Miguel, Thanks so much for the comment. This is a seven year old posting and we did not have the ability to post comments when it was originally posted online. We have made an update on What’s That Bug? and your comment is greatly appreciated.

Ah, there is also needed the size and the picture of its face so we can see the eye arrangement, depending on that it could also be pisauridae, but I stay in Ctenidae..

This is a female Cupiennius sp. wandering spider.

Perhaps surprisingly, this ubiquitous large spider of the Mindo area appears to be undescribed to species level.

Although one is indeed best advised to exercise caution in the presence of large ctenids, members of the genus Cupiennius are not known to be dangerously venomous (Barth, 2002). By way of confirmation, my girlfriend, Shannon Bowley, managed to be bitten by a mature female of this Mindo species in 2013 – she felt only mild effects, equivalent to a bee sting.

Thanks for this valuable information.

I’m planning a trip to Ecuador and I’m fearing these spiders. Do they get in houses? Any tips to keep them out, so I can sleep at night?

Leave a Comment Cancel reply

Save my name, email, and website in this browser for the next time I comment.

Notify me of followup comments via e-mail. You can also subscribe without commenting.

A home for Tarantula, Spider, Scorpion and Arachnid Enthusiasts.

Brazilian Wandering Spider size: how big do they get?

As an avid arachnid enthusiast, my fascination with these eight-legged creatures knows no bounds. There’s something utterly captivating about their intricate web-weaving capabilities, their exceptional survival strategies, and of course, their range of sizes and appearances. But amongst this vast collection of species, one has particularly piqued my interest – the Brazilian Wandering Spider.

Arguably one of the most notorious names in the world of arachnids, the Brazilian Wandering Spider, holds a firm position at the pinnacle of spider folklore. Their reputation extends far beyond their native tropical South American rainforests, captivating the minds of arachnologists and laymen alike around the globe.

What sets this spider apart in the arachnid world? It’s not just its venomous nature – it’s also its astonishing size. The dimensions of the Brazilian Wandering Spider are a spectacle in themselves.

How big are Brazilian Wandering Spiders?

Picture this: a spider so large that it could comfortably cover an average dinner plate. That’s right – the Brazilian Wandering Spider can grow to an impressive leg span of up to 7 inches , or approximately 18 cm, with females tending to be larger than the males.

To put this into perspective, that’s around half the size of an average adult human’s face! The body length of these spiders can reach up to 2 inches, or around 5 cm. This considerable size, coupled with their highly potent venom, makes them a formidable presence in their habitats.

But as an arachnid hobbyist, the allure of the Brazilian Wandering Spider’s size lies not in its potential threat, but in the fascinating evolutionary adaptations that have allowed it to grow to such an impressive scale.

The advantage of large size for a cursorial hunter

From an evolutionary standpoint, the Brazilian Wandering Spider’s significant size offers multiple advantages. For starters, their considerable size allows them to subdue and consume a larger variety of prey, including insects, other spiders, and sometimes even small amphibians and reptiles. This dietary diversity is a testament to their adaptability and survival prowess.

However, the size of these spiders is not solely an indicator of their hunting prowess. It also reveals a fascinating aspect of their biology. The Brazilian Wandering Spider, unlike some smaller spider species, does not weave a web to capture its prey. Instead, as their name suggests, they wander in search of food, relying on their size and strength to overpower their prey. This is called cursorial hunting .

Their size also plays a crucial role in their mating rituals. Male Brazilian Wandering Spiders, despite being smaller than their female counterparts, engage in a daring dance of danger during mating. They utilize their size and strength to lift the larger female’s body to prevent her from eating him – a gruesome but prevalent behavior known as sexual cannibalism in the arachnid world.

Size relates to the biology of the Wandering Spider

The Brazilian Wandering Spider’s size, therefore, is much more than just an intimidating factor. It’s a key to understanding their hunting methods, their mating rituals, and their overall survival strategy. Each inch adds to the mystique of these extraordinary creatures and deepens our understanding of the diverse world of arachnids.

The Brazilian Wandering Spider is undeniably a creature of fascination. From its substantial size to its potent venom, it’s a spider that demands respect. Whether you’re an arachnid hobbyist like me, or just someone intrigued by the wonders of the natural world, the Brazilian Wandering Spider offers a fascinating insight into the awe-inspiring world of arachnids.

Final thoughts…

In conclusion, the world of spiders is as vast and varied as the creatures themselves. The Brazilian Wandering Spider, with its impressive size, offers a fascinating window into this world. It’s a testament to the incredible diversity of life on Earth and a reminder of the intricate balance that governs our ecosystems.

My fascination with spiders continues to grow, much like the impressive size of the Brazilian Wandering Spider. And while their size may be a source of fear for some, for me, it only fuels my passion and curiosity about these fascinating creatures. After all, understanding is the first step to appreciation, and these giant spiders deserve nothing less.

Whether you’re a fellow arachnid enthusiast or just passing by, I hope this deep-dive into the size of the Brazilian Wandering Spider has sparked your curiosity. So the next time you hear about this giant spider, remember, there’s more to its size than meets the eye!

If you’d like to learn more about these spiders, check out my article on Brazilian Wandering Spider facts .

FAQ related to Brazilian Wandering Spider Size

How bad is a brazilian wandering spider bite.

A Brazilian Wandering Spider bite is considered a medical emergency. If you have a suspected or confirmed bite, your first move should be to contact the emergency services. Upon reaching you, they will determine whether to administer antivenom, and how to proceed with treatment. Fortunately, deaths are very rare, and the antivenom is widely available in South America.

Is a banana spider the same as a Brazilian wandering spider?

The common name of “Banana Spider” is used for a few species, not just the Brazilian Wandering Spider. For example, the Golden Silk Orb Weaver is often referred to as a banana spider, despite being completely unrelated. If you wish to find out if a spider is venomous, always rely on its scientific, or binomial name, rather than common names.

Where do Brazilian wandering spiders hide?

Brazilian Wandering Spiders are nocturnal hunters, and generally prefer to shy away from daylight. This means they often hide in the shadiest places they can find, either indoors or outdoors. For example, under a log or in leaf litter would be a perfect place outdoors. Indoors, they will try to hid behind or under furniture, or in dark corners.

What is the world’s biggest Brazilian wandering spider?

There are around 40 species of Wandering Spider, but the largest, and most infamous are those that we call the Brazilian Wandering Spider. This name is in fact commonly ascribed to two species: Phoneutria nigriventer and Phoneutria fera. Both of these spiders can reach at least 6 inches (15cm) in legspan.

Related Posts:

Leave a Comment Cancel Reply

Your email address will not be published. Required fields are marked *

Save my name, email, and website in this browser for the next time I comment.

• Check availability
• Book online
• Posada Amazonas
• Refugio Amazonas
• Tambopata Research Center
• Jungle Lodge Comparison Chart
• What to bring
• All You Need To Know About Travel To Tambopata
• Tambopata National Reserve
• The Wildlife
• Amazon Tours
• Accessible Amazon – Virtual Experiences
• Canopy Tower
• AmazonCam Tambopata – Citizen science
• Chuncho Macaw Clay Lick
• Discovering New Species
• See All Activities
• Travel offers
• Wired Amazon Program
• Ese Eja Native Community of Infierno
• Traveling sustainable

FACTS ABOUT Wandering spider

Brazilian wandering spider

Phoneutria genus.

BACK TO ALL WILDLIFE

Did you know? Our Rainforest Expeditions guests find the relatives of these shy, eight-legged creatures on night hikes!

Brazilian wandering spider fun facts.

• Most toxic spider venom is known : The venom of at least one species of Phoneutria is much more toxic than the Black Widow Spider and can easily kill mice with one bite. Human fatalities in Brazil are an extremely rare occurrence.
• No web : Unlike many other spiders, the wandering spiders don’t build webs but actively search for their prey.
• The banana spider : Wandering spiders occasionally turn up in shipments of bananas! One such stowaway actually bit a man in England in 2005. He survived but spent a week in the hospital!
• Understory predator : These spiders are active, aggressive predators that feed on large insects, small lizards, mice, and frogs.

How to plan your Amazon jungle travel to see Brazilian Wandering Spiders and other jungle wildlife

• Go on a night hike: These large spiders are most active at night. We have nightly hikes in the rainforest with trained guides so you can see wandering spiders and other nocturnal jungle animals.
• Make science happen with AmazonCam: even if Amazon Travel is not in your short-term plans, you can connect with Amazon wildlife!
• And of course, if you´re thinking (or even dreaming) of Amazon jungle Travel, drop by to chat with the Amazon Travel experts . We will help you get there.

OTHER EXPLORERS HAS ALSO CHECKED

• SIDE NECKED TURTLE (Podocnemis unifilis) SIDE NECKED TURTLE (Podocnemis unifilis) Height 80 cmts Weight 4.5 kgs Lodge Tambopata Research Center, Posada Amazonas
• SCARLET MACAW (Ara Macao) SCARLET MACAW (Ara Macao) Height 90.70 cm Weight 1 kg Lodge Tambopata Research Center
• CHESTNUT-FRONTED MACAW (Ara severa) CHESTNUT-FRONTED MACAW (Ara severa) Height 40-50 cm Weight 300 - 390 gr Lodge Tambopata Research Center
• SPIDER MONKEY (Ateles chamek) SPIDER MONKEY (Ateles chamek) Height 90 cm Weight 7-10 kgs Lodge Tambopata Research Center

{$MOD_TEXTOINTRO_01$}

{$MOD_GRILLAWILDLIFE$}

What our guest say

• Valuation Excellent

Mo Bashagha Feb 2024

Nelehkoh9 feb 2024, jen101382 feb 2024, vigs feb 2024, shovan bhattacharrya feb 2024, shovan b feb 2024, michele feb 2024, rahul s jan 2024, amina aziz jan 2024, voyage51682856837 jan 2024, sundar v jan 2024, tina a jan 2024.

A truly magical experience. It’s set in a completely unique location in the middle of the jungle with no other lodges around. The guide we had (Jarinson) was the best, so so knowledgeable, experienced and lovely to spend the time with. The lodge itself is beautiful and food was great too. Would highly recommend. Read all

Interesting bus and river boat rides bring you to Posada Amazonas on the Tambopata River. It’s managed by the Ese Eja indigenous community. Luis M., our guide, helped us observe birds and learn about plants and other animals. A canopy tower, clay lick and oxbow lake are highlights. All personnel are extremely kind and researchers give short lectures in the evening. The food is delicious! Excellent mosquito netting in rooms! Read all

Wow where do I even begin. This was one of the most unique and special experiences we've ever had. You are smack dab in the middle of nowhere, deep in the Amazon jungle. Surrounded by plants and animals of all kinds and some of the most breathtaking scenery you can imagine. And in the company of scientists doing conservation work all around you. The facility is gorgeous beyond belief. Everything is so thoughtfully prepared, from water servers everywhere to comfy couches to hammocks to raised walkways so you can pad around barefoot without feeling like you're going to step on a scorpion. The meals are positively gourmet. They have delicious fresh juice out for you when you come back from your hikes. We spend 2 days there and I genuinely feel like I saw the jungle. 2-3 excursions a day with a private guide to show you everything from plants to birds to animals to a very tall tower where you can watch the sunset over the canopy. We only had 3 people in our group but I believe there can be up to 7. You have the same guide your whole trip so you really get to know them. Johan was our guide and he was wonderful. And I do believe the number of excursions/type/length can be adjusted based on your groups physical ability. The rooms are beautiful and comfy and they help you prepare mosquito netting at night so you can be comfortable. There's a full bar, in the middle of the rainforest. At night there are short (30 min) lectures you can go to if you want where the scientists talk about their work. Fascinating. We loved that the center was very eco conscious and put a lot of effort into being low impact while also offering a truly luxurious experience. That being said. Do yourself a favor and read the web site. This experience will not be for everyone. Ppl posting negative comments here about the heat, bugs, open rooms, etc did not do their homework. You are far, far inside pristine Amazon jungle. It is hot, humid, rainy, and there are bugs. You can expect to use your lovely shower a few times a day to cool off. Fortunately you have lots of down time during the heat of the day to rest under the ceiling fan in your room. There were lots of grasshoppers, moths etc around our room but honestly we didn't have many issues with biting insects or mosquitos despite visiting during the rainy season (we did use bug spray). Your room has one open wall that faces nature. Yes, technically than means someone could see you walking around naked in your room, but you are 15 feet up in the air and facing directly into thick jungle, there is no one out there besides birds and monkeys to see you. They do cut the brush back so nothing except maybe some birds could get into your room. The sounds are magical at night and during the day. It's hot especially if you go during the rainy season as we did. If you hate bugs or can't handle life without air conditioning, this is not for you. Truthfully we had no issues sleeping. Ventilation is another big benefit of the one open wall. Think of this as the glampiest glamping you'll ever do and you will not be disappointed. Read the web site and reviews thoroughly and you won't be surprised. Being good hiking shoes, a lightweight waterproof jacket, refillable water bottle, a hat, binoculars (you can rent them too), bug spray, and sunscreen. Lightweight long sleeved shirts and long pants are essential bc you want to be covered so you won't get bitten or in case you brush up against a nasty plant. We saw monkeys, macaws, capybaras, owls, a caiman, and some cool jungle bugs. The guides even have a telescope you can use to both look at the animals and take great pictures with your phone. once in a lifetime experience in one of the most special and precious ecosystems in the world. Worth every penny and honestly we felt the price was cheap considering what you get. The highlight of our trip to Peru. Thank you so much. Read all

It was our first time in the rainforest and we wouldn't do it any other way. The boat rides on the Madre de Dios and the walks through the rainforests were both exciting and peaceful. Exciting when we spotted wildlife and peaceful as we settled in to hear the sounds and sights of the forest. The lodge was comfortable and the food was great. The research talks in the evenings were a great pre-dinner ritual for us. Do check them out. Saul was an amazing guide - friendly, fun, knowledgeable and very tuned to the rainforest. He could pick up on slight sounds and movements and helped us spot some great wildlife. Read all

TRC is up close with nature deep inside the Peruvian Amazon. If you are a fan of wildlife and love being in the nature t his is the place for you. This is an eco lodge, so come with similar expectations. The sound of macaws flying over would make your day. TRC is doing some amazing work on Macaw conservation, the visits to the clay lick would be special. During your journey you would be able to spot a number of animals, birds. Do not miss the guided walks, you would really see a lot of monkeys. If you are a photographer, invest in a good zoom lens ( it will pay you back). The other thing is the amazing staff that take care of you. The food is among the very best I tasted in Peru and they really pamper you. So make sure you walk a lot to burn the additional calories 🙂 Starting from the booking to the pickup in the airport everything is meticulously planned. From Mariella of the booking team, Juan Carlos the guide and Tania, our manager in the hotel everyone was fantastic. Ultimately spotting wild animals is a lot of luck, but this would be a great experience. Rooms: Rooms are large, spacious with wide open balconies, but no monkeys come in ( they strictly don't encourage wildlife feeding). Nearby activities: Lots of them, morning hikes, afternoon hikes, sunset cruise Walkability: You need to walk a bit, so you need to be mobile Food & drinks: Awesome Buffet, great food. Read all

TRC is up close with nature deep inside the Peruvian Amazon. If you are a fan of wildlife and love being in the nature t his is the place for you. This is an eco lodge, so come with similar expectations. The sound of macaws flying over would make your day. TRC is doing some amazing work on Macaw conservation, the visits to the clay lick would be special. During your journey you would be able to spot a number of animals, birds. Do not miss the guided walks, you would really see a lot of monkeys. If you are a photographer, invest in a good zoom lens ( it will pay you back). The other thing is the amazing staff that take care of you. The food is among the very best I tasted in Peru and they really pamper you. So make sure you walk a lot to burn the additional calories 🙂 Starting from the booking to the pickup in the airport everything is meticulously planned. From Mariella of the booking team, Juan Carlos the guide and Tania, our manager in the hotel everyone was fantastic. Ultimately spotting wild animals is a lot of luck, but this would be a great experience. Read all

The immersion experience was great. Be prepared though to feel hot and full of bug spray much of the time. Wear hiking p ants. Not Lulu leggings (too hot). The guides are great and you can do as much or as little as you want. We saw a lot of animals. Read all

Posadas Amazonas is an eco-friendly lodge that makes a huge commitment to preserving the rainforest for generations to c ome. The food served (breakfast, lunch and dinner) is fresh and delicious! The staff is extremely knowledgeable and caring. A special shoutout to the lodge manager Cesar for ensuring that we had packed meals for the onward journey when we were delayed by a rain storm. The main attractions apart from the abundant wildlife are the canopy tower and nearby Oxbow lake. The canopy tower gives a great 360 degree view of the rainforest and we were fortunate to see monkeys and macaws from the vantage point. At the Oxbow lake we saw river otters and Caimans and we were able to catch a piranha fish as well. Overall, Posada Amazonas is a great lodge owned and operated by the native communities located in the amazing Amazon rainforest. Read all

I had an amazing time here. I was traveling solo and did 4 days/3 nights through Inca Expert Travel. It was an unforgett able experience! I opted for a group tour for the excursions and am glad I did. Our guide, Boris, was fantastic and made sure we saw as much wildlife as possible. I can't say enough good things about him. The rest of the staff were also very kind. I enjoyed the large variety of fresh food at each meal. I felt safe and well-taken care of the entire time. Read all

TRC was an amazing place to stay. Being in the Amazon rainforest was truly like no other trip I have or will ever take. From arrival we were greeted by staff with cool drinks, warm welcomes. Beautiful main area w bar, sofas, tables,games. Our guide Fernando was SO knowledgeable, interesting, enthusiastic,. We already miss his smile and laugh 🙂 We did every excursion available over our 4 days there. The night hike turned out to be our favorite as we spotted the wandering spider then and many other intersting moths/insects. January is hot and humid, so yes expect to be wet,sweaty. Pack extra bag for wet stinky clothes ... Be ok w insects as no 4th wall in your rooms so at night mosquitio nets do a good job preventing bugs in your bed but when you wake up to use bathroom excpect moths, grasshopppers etc in room. All harmless!! TRC does provide nice rainboots for wet swampy treks.. Read all

A perfect experience in the Amazon. Harry, our guide, made the entire experience very memorable. The food is good, the b ar is expensive but good and the support staff were excellent. Tanya, the manager, took care of all our needs and was very supportive the entire stay. Please remember that the rooms are open to the nature and therefore comes with its pros and cons. You wake up with the nature including the mosquitos 🙂 The experiences offered by the Refugio is fantastic and provides a great exposure to the Amazon. Dont miss the Macaw trip. It is just magical. Read all

This is a once-in-a-lifetime experience! From the getgo, it was easy to coordinate our 4-day stay at Tambopata. The book ing was all done via email with quick responses, there was clear communication leading up to our stay and even upon arrival the staff was extremely organized (incl. taking our bags and making sure they arrived to our rooms). During our stay the staff was all very friendly and accomodating, the lodge was always clean (actually impressively clean given it is located in the jungle), and the food was great and plentiful. By far the best part of our stay was our tour guide, Saul. Upon arrival, Saul helped us pick our activities based on what animals we wanted to see (using his 10 years of experience). He worked on timing to make sure we can see as much as possible, was extremely knowledgable on the rainforest, the plants, the insects and the animals and noticeably put in the extra effort to find animals. You can tell that he truly loves his job and was always equally as excited to see the animals as us, even though he has seen them thousands of times. He helped us find monkeys, birds, countless insects, lizards, capybaras ect. I am 100% confident our experience wouldn't be the same without him. He even helped us capture these cool photos with only our iphone!! If you are planning to stay are TRC, I would highly recommend asking for Saul as a tour guide, if possible. Read all

Join To Our Newsletter

Search in Site

Animals · Reptiles & Amphibians

Brazilian Wandering Spider

April 30, 2013 by shamsul

Common name: Brazilian Wandering Spider

Type: Reptiles & Amphibians

Family: Ctenidae

Range: When you consider about the range of this Brazilian wandering spider, you can see this in the forests of Costa Rica and also throughout the South America including the places Colombia, northern Argentina, Paraguay, Guiana, Brazil, Peru, Ecuador, Bolivia, east of Anders and Venezuela. You can also see some of the species in the Amazon. This spider also introduced to Uruguay and Chile.

Size: The spiders of this kind normally have the capability to grow with the leg span of 13 to 15cm or 5.1 to 5.9 inches. They have the body length as ranged from 17 to 48 mm. The body size may be varied depending on the species and the largest species would have longest body. This species has the greatest body weight compared to other spiders of this group.

Diet: The adult Brazilian wandering spider prefers to eat mice, crickets, small lizards and crickets. The spiderlings would probably eat the pinhead crickets and flightless fruit flies. Average lifespan: The average lifespan of this spider is normally one to two years

Habitat: The main reason for calling this spider as wandering one is that, it would probably wander out the jungle floor apart from concentrating on maintaining a web and residing in a lair. Because of this reason, it is also treated as the dangerous one by most of the people. When you consider about this spider, it usually searches for the dark and covered places for hiding during daytime in the case of densely populated place. It also prefers to hide within boxes, houses, cars, log piles, clothes and boots. This would probably cause an accident sometimes, when people disturb them. It is also known as banana spider, since it prefers to find within the shipments of bananas. Because of this reason, the large spider that appears on the bunch of bananas would necessary to be treated with efficient care.

Breeding/Reproduction: When you consider about the reproduction of this spider, it would probably reproduce the eggs as packed into silk bundles those are treated as the egg sacs. In most of the cases, male spider would make the mating with the timely departure in order to escape before the female return to the normal position. You can identify the gender of this spider with the help of the end of their palps, through which the male spider would probably available with the swollen bulbs.

At the time of breeding season, the female spider would store the sperm inside it that too in the chamber and it uses it at the time of egg-laying process. When the sperm gets contacted with the female egg, it would get started with the fertilization. After that particular period, it would probably release out the young spiders.

When you consider about this genus spider, it is normally differed from other genus with the help of presence of a dense brush of fine hairs called as prolateral scopulae that are presented on the pedipalp tibiae along with the tarsi in both sexes. But, there are some species in this spider variety that would probably lack red hairs on the chelicerae because of the unreliable and efficient identification feature. When you go through the identification of this species, it would probably be done with the help of dark linear stripes or stripes that are presented on the frontal palps along with the presence of single thin black line running on dorsal carapace of anterior-posterior thing. Other features of this spider included with the strong ventral marking available on the underside of the legs that too with the contrasting talk mid-segments options and lighter joints. Apart from that, the pattern on the ventral of the abdomen of the spider is available with the several rows of black dots or in the color of red.

There is also an efficient and reliable character available with Brazilian Wandering Spider species for indication and that is the defensive posture presented with front legs that too as high one. It is also available with the correct color patterns to provide attractive look. At the time of defense display, it would probably life up the body into an erect position along with first two pairs of legs as lifted high in order to reveal the conspicuous black or light banded pattern available on the leg underside, and also the spider prefer to sway from side to side with cocked positioned hind legs.

When you consider about the web of Brazilian Wandering Spider , most of the spiders would spin their web with the help of silk, thin and strong protein thread like stand extruded from their body. It would probably find at the end of the abdomen that too as the spinnerets. Most of the species prefer to use this web for trapping the insects and some species like Brazilian wandering spider prefer to use it for their living and also to hunt. The silk emitted from its body would probably help for climbing, wrap prey, form smooth walls for the purpose of burrows, hold sperm temporarily, build egg sacs and also for some other efficient applications.

Since this spider is normally available in the forests of Costa Rica , when you go for the trip to that place then it would be efficient to go for the spider watching along with other animals and birds. They can found in Braulio Carrillo National Park, Chirripo National Park, Juan Castro Bianco National Park, Monteverde Cloud Forrest Reserve, Poas Volcano National Park and Santa Elena Cloud Forest Reserve. This would probably provide you with the option to get believed about the advantages and disadvantageous things.

It is more helpful for the students who want to go through the research regarding the dangerous spiders like Brazilian Wandering Spider . Most of the species related to this spider would probably be treated as the one to threat humans, not because of the toxicity and also because of the sufficient quantity of venom they produce.

Share this:

Comments are closed.

An official website of the United States government

The .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

• Publications
• Account settings

Preview improvements coming to the PMC website in October 2024. Learn More or Try it out now .

• Advanced Search
• Journal List
• Front Mol Biosci

Holistic profiling of the venom from the Brazilian wandering spider Phoneutria nigriventer by combining high-throughput ion channel screens with venomics

F. c. cardoso.

1 Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia

2 Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia

A. A. Walker

M. v. gomez.

3 Department of Neurotransmitters, Institute of Education and Research, Santa Casa, Belo Horizonte, Brazil

Rodrigo Ligabue-Braun , Federal University of Health Sciences of Porto Alegre, Brazil

Luiza Gremski , Federal University of Paraná, Brazil

Associated Data

The datasets presented in this study can be found in online repositories. The name of the repository and accession number are ProteomeXchange PRIDE repository; PXD037904.

Introduction: Spider venoms are a unique source of bioactive peptides, many of which display remarkable biological stability and neuroactivity. Phoneutria nigriventer , often referred to as the Brazilian wandering spider, banana spider or “armed” spider, is endemic to South America and amongst the most dangerous venomous spiders in the world. There are 4,000 envenomation accidents with P. nigriventer each year in Brazil, which can lead to symptoms including priapism, hypertension, blurred vision, sweating, and vomiting. In addition to its clinical relevance, P. nigriventer venom contains peptides that provide therapeutic effects in a range of disease models.

Methods: In this study, we explored the neuroactivity and molecular diversity of P. nigriventer venom using fractionation-guided high-throughput cellular assays coupled to proteomics and multi-pharmacology activity to broaden the knowledge about this venom and its therapeutic potential and provide a proof-of-concept for an investigative pipeline to study spider-venom derived neuroactive peptides. We coupled proteomics with ion channel assays using a neuroblastoma cell line to identify venom compounds that modulate the activity of voltage-gated sodium and calcium channels, as well as the nicotinic acetylcholine receptor.

Results: Our data revealed that P. nigriventer venom is highly complex compared to other neurotoxin-rich venoms and contains potent modulators of voltage-gated ion channels which were classified into four families of neuroactive peptides based on their activity and structures. In addition to the reported P. nigriventer neuroactive peptides, we identified at least 27 novel cysteine-rich venom peptides for which their activity and molecular target remains to be determined.

Discussion: Our findings provide a platform for studying the bioactivity of known and novel neuroactive components in the venom of P. nigriventer and other spiders and suggest that our discovery pipeline can be used to identify ion channel-targeting venom peptides with potential as pharmacological tools and to drug leads.

Introduction

Venomous animals are a highly adapted group of organisms whose evolutionary success excelled with the emergence of venom. Spider venoms, in particular, are rich in peptide knottins specialized in modulating, often with high potency and selectivity, voltage-gated ion channels that regulate the physiology of neuronal, muscular and cardiac systems ( Cardoso and Lewis, 2018 ; Cardoso, 2020 ). Although such effects can be deleterious to envenomated animals, venom components can be tailored to selectively modulate ion channels in pathways of complex diseases such as chronic pain, motor neuron disease, and epilepsy. This has been demonstrated for numerous spider venoms ( Smith et al., 2015 ; Cardoso and Lewis, 2018 , 2019 ), including the venom of the infamous South American ctenid spider Phoneutria nigriventer , often referred as Brazilian wandering spider, banana spider or “armed” spider ( Peigneur et al., 2018 ). Besides its clinical relevance due to frequent envenomation cases in Brazil, with approximately 4,000 cases per year ( Isbister and Fan, 2011 ; Gewehr et al., 2013 ), P. nigriventer venom contains peptides that have therapeutic effects in a range of disease models including chronic pain ( Pedron et al., 2021 ; Cavalli et al., 2022 ), Huntington’s disease ( Joviano-Santos et al., 2022 ), glaucoma ( da Silva et al., 2020 ) and erectile dysfunction ( Nunes da Silva et al., 2019 ).

Initial studies of P. nigriventer venom employed fractionation via gel filtration and reversed-phase chromatography to separate the venom into five distinct groups of peptides based on their molecular weight and hydrophobicity properties; these groups were named PhTx1 to PhTx5 ( Peigneur et al., 2018 ). PhTx1–4 comprise cysteine-rich peptides that are active on voltage-gated calcium (Ca V ), sodium (Na V ) and potassium (K V ) channels, while PhTx5 is comprised of short linear peptides, with a total of 34 peptides identified ( Peigneur et al., 2018 ). Proteotranscriptomic studies of P. nigriventer venom revealed additional peptides with high similarity to those previously described, but very few have been characterised pharmacologically ( Cardoso et al., 2003 ; Richardson et al., 2006 ). This represents an obstacle to the exploration of the therapeutic potential of P. nigriventer venom.

Advances in venom-peptide research have yielded high-throughput cellular screens for the discovery and pharmacological characterisation of naturally occurring molecules with activity at ion channels and receptors in physiological pathways ( Cardoso et al., 2015 ; Cardoso et al., 2021 ). These methods require only a small amount of venom compared to more traditional methods and allow the identification of therapeutically relevant peptides in the early stages of the screening. Besides drug development applications, these same bioassays can assist in unravelling the bioactivity of crude and fractionated venoms from biomedically relevant venomous animals to support studies of evolution and antivenom development, but much work remains to be done in this field.

This study aimed to provide a proof-of-concept in applying high-throughput cellular screens for multiple neuronal ion channels along with proteomic studies of fractionated venom to rapidly characterise spider venoms in terms of bioactive components. It was anticipated that such a pipeline would support envenomation and evolutionary studies and the development of therapeutics from animal venoms. The venom of P. nigriventer was selected as a model system due to its medical relevance, the considerable number of therapeutically relevant peptides already uncovered in the venom, and the wide knowledge base available. Our approach enabled identification of potent modulators of voltage-gated ion channels which were classified into four families of neuroactive peptides based on their activity and structures. In addition to the previously characterised neuroactive peptides in the P. nigriventer venom, we identified 27 additional cysteine-rich venom peptides in which neuroactivities are underexplored. This work contributes to the on-going discovery and structure-function characterisation of spider-venom peptides. Moreover, our bioassay pipeline can be used to guide future research into the discovery of venom peptides that modulate the activity of ion channels, and their development as pharmacological tools and drug leads.

Materials and methods

We applied a holistic approach combining methods in high throughput screens for ion channels, venom proteome, venom gland transcriptome and modelling of peptides as described in Figure 1 .

Flowchart of the venom peptide discovery pipeline applied in this study. Expanding from the traditional assay-guided fractionation, we applied HTS bioassays to characterize the pharmacology of venom peptides on multiple ion channels, followed by the identification of peptide masses and primary sequences using proteome and transcriptome. Ultimately, the three-dimensional structure of venom peptides was determined using in silico molecular modelling.

Cell culture

The human neuroblastoma cell line SH-SY5Y was maintained at 37 ° C in a humidified 5% CO 2 incubator in Roswell Park Memorial Institute (RPMI) medium supplemented with 15% foetal bovine serum (FBS) and 2 mM L-glutamine. Replicating cells were sub-cultured every 3–4 days in a 1:5 ratio using 0.25% trypsin/EDTA.

Venom fractionation

Crude venom milked from male and female specimens of P. nigriventer was kindly provided by Prof. Marcus Vinicius Gomez from the Institute of Teaching and Research of Santa Casa de Belo Horizonte, Belo Horizonte, Brazil. Venom (lyophilised, 1 mg) was dissolved in 100 μL Milli-Q water containing 0.05% trifluoroacetic acid (TFA) (Auspep, VIC, AU) and 5% acetonitrile (ACN) and centrifuged at 5,000 × g for 10 min to remove particulates. Venom was fractionated by reversed-phase high performance liquid chromatography (RP-HPLC) using a C18 column (Vydac 4.6 mm × 250 mm, 5 μm, Grace Discovery Sciences, United States) with a gradient of solvent B (90% ACN in 0.045% TFA) in solvent A (0.05% TFA). The gradient was 5% B for 5 min, followed by 20%–40% solvent B over 60 min at a flow rate 0.7 mL min −1 . Peaks were collected every minute, with fraction 1 eluted between 1 and 2 min and so on for the other fractions. Venom fractions were lyophilised before storage at –20°C.

Calcium influx assays

Venom fractions were screened for neuroactivity at human (h) Na V , Ca V 1, Ca V 2 and the α7 subtype of the human nicotinic acetylcholine receptor (nAChR-α7) as previously described ( Cardoso et al., 2015 ). Briefly, SH-SY5Y cells were plated at 40,000 cells per well in 384-well flat clear-bottom black plates (Corning, NY, United States) and cultured at 37 ° C in a humidified 5% CO 2 incubator for 48 h. Cells were loaded with 20 μL per well Calcium 4 dye (Molecular Devices) reconstituted in assay buffer containing (in mM) 140 NaCl, 11.5 glucose, 5.9 KCl, 1.4 MgCl 2 , 1.2 NaH 2 PO 4 , 5 NaHCO 3 , 1.8 CaCl 2 and 10 HEPES pH 7.4 and incubated for 30 min at 37 ° C in a humidified 5% CO 2 incubator. For the hCa V 1 assay, the dye was supplemented with 1 μM ω-conotoxin-CVIF (CVIF) to inhibit Ca V 2, and in the hCav2 assay the dye was supplemented with 10 μM nifedipine to inhibit Ca V 1. For the nAChR-α7 assay, the dye was supplemented with PNU-120596 (Sigma-Aldrich), a positive allosteric modulator of nAChR-α7. Venom fractions were assayed in singleton for each ion channel tested. Fluorescence responses were recorded using excitation at 470–495 nm and emission at 515–575 nm for 10 s to set the baseline, then 300 s after addition of 10% venom fraction serial diluted at 1, 1:10, and 1:100, and for a further 300 s after addition of 50 μM veratridine for hNa V , 90 mM KCl and 5 mM CaCl 2 for hCa V, and 30 μM choline for nAChR-α7.

Venom fractions eluting between 10 and 45 min on RP-HPLC were analysed by mass spectrometry to investigate the masses and primary structures of their peptide components. Native mass determinations were carried out with 20% of each fraction dried by vacuum centrifuge and resuspended in 20 μL 1% formic acid (FA), followed by analysis using by liquid chromatography/tandem mass spectrometry (LC-MS/MS). For identification of primary structures, 20% of each peptide fraction was reduced and alkylated by adding 40 μL of reagent composed of 4.875 mL ACN, 4.5 mL ultrapure water, 0.5 mL 1M ammonium carbonate pH 11.0, 100 μL 2-iodoethanol and 25 μL triethylphosphine, and incubating for 1 h at 37°C. Samples were speed dried in a vacuum centrifuge, and digested with 40 ng/μL trypsin in 50 mM ammonium bicarbonate pH 8.0 and 10% ACN overnight at room temperature. Trypsin was inactivated by adding 50 μL solution containing 50% acetonitrile and 5% formic acid (FA), dried in speed vacuum centrifuge, and resuspended in 1% formic acid.

LC-MS/MS samples were loaded onto a 150 mm × 0.1 mm Zorbax 300SB-C18 column (Agilent, Santa Clara, CA, United States) on a Shimadzu Nano LC system with the outflow coupled to a SCIEX 5600 Triple TOF (Framingham, MA, United States) mass spectrometer equipped with a Turbo V ion source. Peptides were eluted using a 30 min gradient of 1%–40% solvent B (90% ACN/0.1% FA) in solvent A (0.1% FA) at a flow rate of 0.2 mL/min. For MS1 scans, m/z was set between 350 and 2,200. Precursor ions with m/z 350–1,500, charge of +2 to +5, and signals with >100 counts/s (excluding isotopes within 2 Da) were selected for fragmentation, and MS2 scans were collected over a range of 80–1,500 m /z . Scans were obtained with an accumulation time of 250 ms and a cycle of 4 s.

A database of possible peptide sequences produced in P. nigriventer venom glands was compiled using a published venom-gland transcriptome ( Diniz et al., 2018 ), from which open reading frames (ORFs) longer than 30 amino acids were identified and translated by TransDecoder. A list of 200 common MS contaminants was added to the translated ORFs, which was used as a sequence database to compare to mass spectral data using the Paragon algorithm in Protein Pilot 2.2 software (AB SCIEX). We report only peptides for which more than two tryptic fragments were detected with >95% confidence, or where one tryptic fragment was detected, and a secretion signal peptide was predicted by SignalP5.0.

Molecular modelling

Venom peptides identified in this study were selected based on their cysteine-rich scaffold and bioactivity, and their three-dimensional (3D) structure were predicted using the AlphaFold 2 algorithm ( Jumper et al., 2021 ). All 3D structures displayed were from unrelaxed models ranked 1 for each peptide prediction. 3D structures were visualised and analysed using PyMol ( Pymol, 2023 ).

Data analysis

Fluorescence traces from singletons were evaluated using the Maximum-Minimum or Area Under the Curve values generated after addition of ion channel activator. Data were normalised against the negative control (PSS buffer control) and positive control (ion channel activator) for each assay and corrected using the response over baseline from 1 to 5 s. No statistical analyses were required in this study.

Screening of P. nigriventer venom fractions

Fractionation of 1 mg of P. nigriventer ( Figure 2A ) crude venom using RP-HPLC produced numerous peaks eluting between 20% and 40% solvent B, and fractions eluting between 11 and 45 min were selected for pharmacological analysis ( Figure 2B ). Screening using the SH-SY5Y neuroblastoma cell line revealed strong modulation of voltage-gated ion channels including both inhibition or enhancement of ion channel activity ( Figure 2C ). Venom fractions eluting between 18 and 34 min showed strong inhibition of Ca V and Na V activity, while fractions eluting between 41 and 45 min strongly activated Ca V 2 channels ( Figure 2C , top panel). At a dilution of 1:10, these inhibitory effects persisted for both Na V and Ca V 2 channels for fractions eluting at 19–20 min and 26–34 min and was absent for Ca V 1 channels ( Figure 2C , middle panel). Fractions eluting from 21 to 25 min showed a clear preference for inhibiting only Ca V 2 channels ( Figure 2C ). Interestingly, at 1:10 dilution, channel activity enhancement was stronger on Na V channels compared to Ca V 2 channels, suggesting potential concentration-dependent synergistic effects of venom peptides modulating both Na V and Ca V 2 channels. At the highest venom dilution of 1:100, persistent inhibition of Na V channel was observed for fraction 20 (F20), while the remaining inhibitory fractions preferentially inhibited only Ca V 2 channels ( Figure 2C , bottom panel). Channel enhancement persisted for Na V channels in fractions eluting from 41 to 45 min. No potent activity was observed against nAChR-α7 at any venom concentration tested. Overall, inhibitory activity was primarily observed for fractions eluting at shorter retention times (i.e., more hydrophilic compounds), while strong ion channel activation was induced by more hydrophobic peptides with longer RP-HPLC retention times.

Fractionation and activity of P. nigriventer venom. (A) P. nigriventer specimen displaying threat posture (photo copyright Alan Henderson, www.minibeastwildlife.com.au ). (B) RP-HPLC fractionation of 1 mg P. nigriventer venom. (C) Ion channel responses calculated from the area under the curve (AUC) after addition of selective activators for fractions 10 to 45, normalized to responses in the absence of venom fractions. (D, E) Representative fluorescence traces of the intracellular calcium responses of SH-SY5Y cells evoked by KCl + CaCl 2 in the presence of venom fractions 26 and 34 for Ca V 1, fractions 19, 26 and 34 for Ca V 2, and fractions 41–45 for both Ca V 1 and Ca V 2 channels. (F) Representative fluorescence traces of the intracellular calcium responses of SH-SY5Y cells evoked by veratridine and in the presence of venom fractions 19, 26 and 34 and fractions 41–45. (G) Representative fluorescence traces of the intracellular calcium responses of SH-SY5Y cells evoked by choline and in the presence of venom fractions 16 and 40 and fractions 41–45. Grey dotted line indicates the KCl + CaCl 2 , veratridine or choline addition.

Fluorescent traces measured upon addition of venom fractions revealed an increase in intracellular calcium ([Ca 2+ ] i ), suggesting that these venom peptides can activate closed channels as well as enhance the responses of these channels opened using pharmacological intervention ( Figures 2D–G ). This was observed for Ca V responses in the presence of 1 μM CVIF (Ca V 2 inhibitor, Figure 2D ) and 10 μM nifedipine (Ca V 1 inhibitor, Figure 2E ). In the absence of Ca V inhibitors, these [Ca 2+ ] i responses resemble the levels of Ca V 1 responses in Figure 2D as observed for F40–F45 applied in the Na V channels assay ( Figure 2F ). The activities of inhibitory fractions were mostly free from initial [Ca 2+ ] i responses upon venom addition, except for weak inhibitors observed in F19 for Na V and F40 for nAChR-α7 ( Figures 2F, G ).

Identification of peptides in P. nigriventer venom fractions

The venom of P. nigriventer has been extensively characterised in terms of composition and bioactivity ( Diniz et al., 2018 ; Peigneur et al., 2018 ), including neuronal ion channel activity and proteomics, but not by using a combined approach. In this study, by combining these approaches, we were able to rapidly identify 58 peptides and proteins in the venom. Due to the complexity of previous nomenclature for P. nigriventer venom peptides, we refer to them here using both the rational nomenclature developed for spider toxins ( King et al., 2008 ) and an identifying number (e.g., PN367) that is linked to a sequence and a list of previously used names in Supplementary Table S1 . Of the 58 identified amino acid sequences, only eight (15%) are peptides that have had their bioactivity reported in previous studies ( Figure 3A , Supplementary Table S1 ) ( Peigneur et al., 2018 ). These included the known neuroactive components μ-CNTX-Pn1a (Tx1) ( Diniz et al., 2006 ; Martin-Moutot et al., 2006 ), κ-CNTX-Pn1a (Tx3-1, PhK V ) ( Kushmerick et al., 1999 ; Almeida et al., 2011 ), ω-CNTX-Pn1a (Tx3-2) ( Cordeiro Mdo et al., 1993 ), Γ-CNTX-Pn1a [Tx4(5-5)] ( Paiva et al., 2016 ), δ-CNTX-Pn1a [Tx4(6-1)] ( de Lima et al., 2002 ; Emerich et al., 2016 ), δ-CNTX-Pn2c (Tx2-5a) ( Yonamine et al., 2004 ), ω-CNTX-Pn4a (Tx3-6) ( Cardoso et al., 2003 ; Vieira et al., 2005 ) and ω-CNTX Pn3a (Tx3-4) ( Dos Santos et al., 2002 ) ( Figure 3B ). Even among these eight peptides, only a few venom peptides have had their molecular pharmacology characterized in detail ( Peigneur et al., 2018 ), or their activities confirmed using recombinant peptides ( Diniz et al., 2006 ; Paiva et al., 2016 ; Garcia Mendes et al., 2021 ).

Estimated levels of peptide/protein venom components identified in fractions F17 to F45, and their respective bioactivity at Na V and Ca V channels and the nAChR-α7. (A) Proportion of known and unknown venom peptides and other venom components detected in this study. (B) Venom peptides with previously reported bioactivity detected in fractions by mass spectrometry and compared to fraction bioactivity at Na V and Ca V channels and the nAChR-α7. (C) Venom peptides detected in fractions classified according to their cysteine framework I to IX ( Diniz et al., 2018 ), and compared to fraction bioactivity at Na V and Ca V channels and the nAChR-α7.

Most of the identified sequences in this study (74%) represent peptides with unexplored bioactivity; 38 (65%) of the 43 peptides identified have cysteine-rich scaffolds typical of spider-venom peptides ( Figure 3C ). Some of these venom peptides, such as PN367 and PN363, have a type I scaffold ( Diniz et al., 2018 ) and are predicted by Alphafold 2 to fold into cystine-knot scaffolds typical of spider-venom peptides ( King and Hardy, 2013 ) ( Figure 4 ). Scaffolds II-VIII either form elaborated cystine-knot folds with extra disulphide bonds, or alternative structures such as for scaffolds III and IV ( Figure 4 ). Novel peptides with high identity with other toxins and not previously described in P. nigriventer venom included: PN367 displaying identity with a Agelena orientalis venom peptide; PN369 displaying identity with a Lycosa singoriensis venom peptide, and PN365 displaying scaffold III and identity with another Lycosa singoriensis venom peptide ( Supplementary Table S1 ). Additional disulphide-rich scaffolds present in P. nigriventer venom include three peptides predicted by the algorithm HMMER to form a thyroglobulin type 1 repeat domain (E < e −17 in each case), one of which has been previously reported as U24-CNTX-Pn1a; peptide PN370 which displays high identity with a peptide found in venom of the scorpion Scorpiops jendeki and is predicted by the algorithm HMMER to form into a trypsin-inhibitor-like cysteine-rich domain (E < 2e −13 ); and the peptide PN376 that is predicted by HMMER to form a fungal protease inhibitor domain (E < e −6 ) ( Supplementary Table S1 ). Additional new scaffolds identified in this study were named following the previous suggested nomenclature ( Diniz et al., 2018 ) as X (CXCC motif, 12 Cys residues: −C−C−C−C−CXCC−C−C−C−C−C−), XI (12 Cys residues: −C−C−C−CXC−CXC−C−CXC−C−C−), XII (11 Cys residues: −C−C−CXC−CXC−C−C−CXC−C) and XIII (10 Cys residues: −C−C−C−C−C−C−CXC−C−C−), and include the peptides PN376, PN372, PN373 and PN375, and PN370, respectively.

Diversity and estimated levels of cysteine-rich scaffolds identified in highly neuroactive RP-HPLC fractions from the venom of P. nigriventer , and their predicted 3D structures. (A) Fractions 18–20 comprised high levels of scaffolds I, II and VIII represented by the 3D structures of PN367, PN105 and PN267, respectively. (B) Fractions 26 and 27 comprised high levels of scaffolds II, and IV, and an undefined scaffold represented by the 3D structures of PN321, PN350 and PN372, respectively. (C) Fraction 34 comprised high levels of scaffolds I, II, and V represented by the 3D structures of PN003, PN292 and PN028, respectively. (D) Fractions 41 and 42 comprised high levels of scaffolds IV and V represented by the 3D structures of PN381, and PN077 and PN031, respectively.

Only 9% of the identified sequences were peptides with two or fewer Cys residues ( Supplementary Table S1 ). F17 contained a peptide (PN361) matching a C-terminally amidated peptide precursor from Araneus ventricosus identified in a genomic study ( Kono et al., 2019 ). This precursor has 70% sequence identify with the prohormone-1 like precursor from the honeybee Apis mellifera (UniProt {"type":"entrez-protein","attrs":{"text":"P85798","term_id":"190360036","term_text":"P85798"}} P85798 ) which is believed to be cleaved to form three short peptides with neuronal activity. Another short peptide, PN366 identified in F18 and F28–F30, matches a neuropeptide in the sea slug Aplysia californica (UniProt {"type":"entrez-protein","attrs":{"text":"P06518","term_id":"113518","term_text":"P06518"}} P06518 ). Larger proteins were also detected in some fractions; for example, F18 and F31 contained a fragment at 58% and 70% total fraction components, respectively, matching a zinc metalloprotease from the nematode Caenorhabditis elegans (UniProt 55112) which contains a peptidase family M12A domain.

Diversity of neuroactive peptides in P. nigriventer venom

The cysteine-rich scaffolds of venom peptides identified in this study were compared to the classification previously proposed for P. nigriventer venom peptides ( Diniz et al., 2018 ) ( Figures 3C , ​ ,4). 4 ). Peptides in fractions displaying inhibitory properties corresponded to scaffolds I, II, IV, V and VIII, as well as unnamed scaffolds, while peptides in fractions with activation properties comprised mostly the scaffold V. All of these scaffolds are inhibitor cystine knot motifs, except for scaffold IV which had the highest level in F26 represented by the peptide PN350.

Neuroactive peptides with greater hydrophilicity (i.e., those with short RP-HPLC retention times) showed pharmacological properties reminiscent of known spider-derived μ-toxins (F17 and F18) and ω-toxins (i.e., inhibition of Ca V 1 and Ca V 2 channels by F19 and F20) ( Figures 2C , ​ ,5A). 5A ). Major components driving those bioactivities were the pharmacologically characterised peptides μ-CNTX-Pn1a, ω-CNTX-Pn1a and ω-CNTX-Pn3a, as well as additional peptides with unknown activity ( Figure 4A ). As the hydrophilicity of the peptides decrease (i.e., peptides with long RP-HPLC retention times), persistent Ca V 2 inhibition was observed with maximum inhibitory activity in F26 and F27, and with the additional peptide ω-CNTX-Pn4a detected in F24 ( Figures 2C , ​ ,3B, 3B , ​ ,5B). 5B ). Interestingly, venom peptides characterized as K V modulators, such as κ-CNTX-Pn1a, were detected in fractions displaying strong inhibition of calcium influx with potential μ- and ω-pharmacology (fractions 26 and 27); it was not clear if the observed bioactivity was associated to the modulation of K V channels, or to other unexplored peptides in these fractions.

Venom peptide content of highly neuroactive RP-HPLC fractions from the venom of P. nigriventer . (A) Identification of the cysteine-rich peptides and proteins in fractions 17–20 displaying potent inhibition of neuronal Na V and Ca V 2 channels. Positively and negatively charged residues are coloured blue and orange, respectively, hydrophobic residues are green, and cysteines are highlighted in grey box. (B) Identification of the peptide and protein content of the fractions 16 and 27 displaying potent inhibition of neuronal Na V , Ca V 1 and Ca V 2 channels. (C) Identification of the peptide and protein content of the fraction 34 displaying potent inhibition of neuronal Na V , Ca V 1 and Ca V 2 channels. (D) Identification of the peptide and protein content of the fraction 34 displaying potent activation of neuronal Na V and Ca V 2 channels. Sequences labelled with a red asterisk (*) at the C-terminal are likely C-terminally amidated.

Neuroactive peptides presenting more hydrophobic structures showed properties of μ and ω-peptides, but with preference for Ca V 2 channels as observed for fraction 34 in which the peptide Γ-Pn1a is the major component, consistent with its previously observed modulation of multiple cation channels ( Paiva et al., 2016 ); and of δ-peptides as observed in fractions 41 to 45, in which major components included the peptides δ-Pn1a and δ-Pn2c ( Figures 2C–F , and Figures 5C, D ). Notably, the main components of some of the most neuroactive fractions are peptides with unexplored bioactivity, e.g., fraction 26 ( Figures 2C , ​ ,4, 4 , ​ ,5 5 ).

Pharmacological groups

Our approach allowed classification of P. nigriventer venom peptides into four major groups based on their bioactivity ( Figure 6 ; Table 1 ). Group 1 is comprised of μ and ω peptides with scaffold type VIII and more hydrophilic properties as they eluted between F17 and F21. As representatives from this group, μ-CNTX-Pn1a and ω-CNTX-Pn3a have a potential “KR electrostatic trap”, a pharmacophore described in spider-venom peptides that modulate ion channels ( Hu et al., 2021 ; Wisedchaisri et al., 2021 ), in their primary and tertiary structures ( Figure 6A ). This pharmacophore is likely composed of residues R61, K67, K70, K71, R74 and R75 in μ-CNTX-Pn1a and residues K54, K56, R59, K65, K70, R71, K73 and K74 in ω-CNTX-Pn3a. Within this group, the ω-CNTX-Pn3a homologue PN319 differs at three positions, making it an interesting candidate for further characterisation.

Pharmacological groups identified in the most active venom fractions highlighting the “KR electrostatic trap” pharmacophore common to spider toxins that modulate the activity of ion channels. (A) Group 1 is represented by μ- and ω-spider-venom peptides with large and complex type VIII scaffold. (B) Group 2 is represented by κ- and ω-spider-venom peptides with type II and VII scaffolds. (C) Group 3 is represented by γ-spider-venom peptides with type V scaffold. (D) Group 4 is represented by δ-spider-venom peptides displaying a type V scaffold. K and R residues located in the C-terminal region of these peptides and grouped on a positively charged face are highlighted in red in the sequences and in red tubes in the corresponding 3D structures. Arrows shows the cysteine-bridge connection forming the cyclic peptide structures predicted for PN028 and PN031.

Pharmacological groups identified in this study with respective pharmacological types, cysteine-rich scaffold types, and representative venom peptides described in the literature. Unexplored peptides within each group are described in Figure 5 and/or Supplementary Table S1 .

Group 2 comprises κ and ω peptides that eluted between F17 and F28, with scaffold types II and VII ( Figure 6B ). As representatives from this group, peptides κ-CNTX-Pn1a, ω-CNTX-Pn1a and ω-CNTX-Pn4a also contain a “KR trap” pharmacophore comprised of residues R20, K23, K34, K35 and K36 for ω-Pn1a; R21, K24, K35 and K36 for κ-Pn1a; and K42, R47, K48, K49, K51, K53 and K54 for ω-Pn4a. In this group, PN107 differs from κ-CNTX-Pn1a by only two residues and is an interesting peptide for further exploration.

Group 3 is comprised of more hydrophobic Γ peptides that eluted in F33–F36 and possess a type V scaffold ( Figures 3C–D , ​ ,6C). 6C ). It is represented by Γ-CNTX-Pn1a with a potential “KR trap” comprising residues K35, R41, K42 and K43. Although Γ peptides modulate N-methyl-D-aspartate (NMDA) glutamate receptors, Γ-CNTX-Pn1a has also been reported as a β-peptide that inhibits Na V channels ( Paiva et al., 2016 ), which agrees with the results from our high-throughput ion channels assays ( Figures 2C–F , ​ ,3). 3 ). Interestingly, Γ-CNTX-Pn1a predicted 3D structure formed a cyclic structure in which the N-terminal cysteine formed a disulfide bridge with C-terminal cysteine ( Figures 4C , ​ ,6C). 6C ). These same fractions contain other ICK peptides including PN003 and PN292 with scaffold types I and II, respectively; their pharmacological targets have not been explored but they likely contribute to the strong inhibition of Ca V channels by F34 ( Figures 2 , ​ ,3, 3 , ​ ,4 4 ).

Group 4 is composed of very hydrophobic δ peptides that elute in F40–F45 and possess a type V scaffold ( Figure 2B , ​ ,6D). 6D ). It is represented by δ-CNTX-Pn1a with potential “KR trap” comprising residues K43, K44, and K45 ( Figure 6D ). In this group we also identified δ-CNTX-Pn2c which differs not only in primary structure but also in the scaffold V tertiary structure by presenting a non-cyclic structure compared to the cyclic structure predicted for δ-CNTX-Pn1a connected by the N- and C-terminal cysteines ( Figures 4D , ​ ,6D). 6D ). Beyond these known peptides, this group comprised interesting unexplored peptides such as PN032 and PN023 showing δ peptide domains and differing from Γ-CNTX-Pn1a by 12 and 11 residues, respectively.

Spiders are one of the most speciose venomous taxa, with >50,000 characterised species (see World Spider Catalog, https://wsc.nmbe.ch/statistics/ ). Their venoms are rich in neuroactive peptides that target a wide range of neuronal ion channels and receptors using mechanisms distinct from those of neurotoxins from other venomous animals such as cone snails and scorpions. The exploration of venom peptides targeting ion channels and receptors provides novel opportunities for the development of pharmacological tools to understand disease mechanisms ( Cardoso and Lewis, 2018 ; Cardoso, 2020 ) as well as provision of leads for development of therapeutics ( King, 2011 ) and bioinsecticides ( Smith et al., 2013 ).

Spiders are classified in two major groups, or infraorders ( King, 2004 ): Mygalomorphae, or so-called “primitive spiders”, includes the family Theraphosidae, or tarantulas, which are the most well studied spider venoms due to the large-size and long lifespan (often >20 years) of these spiders. Araneomorphae, or “modern spiders,” comprise >90% of all extant spider species, including the family Ctenidae in which P. nigriventer resides. Notably, despite their much greater species diversity, araneomorph venoms are underexplored compared to mygalomorphs due to their smaller size and shorter lifespan (typically 1–2 years). Our data, and those of others ( Binford et al., 2009 ; Zhang et al., 2010 ; Diniz et al., 2018 ; Peigneur et al., 2018 ), showed a great diversity of both pharmacological actions and cysteine scaffolds in araneomorph venom, which may have facilitated the highly successful araneomorph radiation. Our data also suggests Araneomorphae’s venoms may be a rich source of unique venom peptides with more diverse structures and pharmacological functions and additional biotechnological and therapeutic applications to Mygalomorphae’s venoms.

The venom from P. nigriventer comprises many exceptional peptides drug leads under development for treating a range of complex neuro disorders ( Peigneur et al., 2018 ). These peptides have been evaluated in pre-clinical models and demonstrated interesting therapeutic efficacy in reverting or preventing conditions for which treatments are limited or unavailable. For example, ω-Pn2a and ω-Pn4a showed efficacy in treating painful neuropathies such as fibromyalgia and chronic post-ischemia pain, respectively ( Pedron et al., 2021 ; Cavalli et al., 2022 ), ω-Pn4a also improved motor movement and neuroprotection in Huntington’s disease ( Joviano-Santos et al., 2022 ). The engineered peptide PnPP-19 derived from the venom peptide δ-Pn2a was efficacious in treating glaucoma ( da Silva et al., 2020 ) and erectile dysfunction ( Nunes da Silva et al., 2019 ). In our study, these therapeutic peptides showed bioactivity at neuronal Na V and Ca V channels, which greatly supports our investigative platform for the discovery of venom peptides useful for the development of efficacious drugs.

Investigative pipelines in venomic studies often focus on the elucidation of venom components based on their structures but lack clear strategies to investigate venom bioactivities ( von Reumont et al., 2022 ). Investigations using fractionated venom ( Cardoso et al., 2015 ; Cardoso et al., 2017 ; Estrada-Gomez et al., 2019 ; Cardoso et al., 2021 ) provides more defined biological functions than using crude venom due to the immense pharmacological diversity of venom, which often contains venom components with opposing activity as well as components that act synergistically ( Raposo et al., 2016 ). Considering the large number of extant spiders and consequently the exceptionally large number of venom components available for investigation, high-throughput (HT) functional bioassays are essential for developing a holistic understanding of venom pharmacology, and they provide a complement to venomic studies.

A recent study by us using HT bioassays to investigate the ion channel targets of Australian funnel-web spider venoms recaptured current taxonomy and revealed potential drug targets to treat severely envenomated patients ( Cardoso et al., 2022 ). In this present study, we also demonstrated the feasibility of applying HT functional bioassays to investigate spider venom components that mediated the activity of voltage-gated ion channels. We were able to capture all known venom components and associated bioactivities using a HT functional assay as well as several new unexplored venom peptides that warrant further exploration. This was achievable only by combining HT bioassays with transcriptomic and proteomic approaches. Although this pipeline provides a robust holistic overview of spider venoms, bioactive components are present in varying concentrations in each fraction, which may affect bioactivity through synergistic effects, and overlook the activity of less abundant components.

The complexity of the cysteine-rich scaffolds in P. nigriventer venom peptides unraveled in this study suggests that further exploration utilising recombinant or synthetic peptides might be challenging but essential, and these could also benefit from modern strategies utilizing HT recombinant expression or chemical synthesis ( Pipkorn et al., 2002 ; Turchetto et al., 2017 ). In tandem with automated whole-cell patch-clamp electrophysiological studies, this will build a pipeline to further investigate known and new peptides in the venom of P. nigriventer and allow selection of candidates with biotechnological potential. The putative “KR trap” pharmacophores identified in those venom peptides warrants further exploration of the structure-function relationships of the diverse pharmacological groups found in the venom of P. nigriventer.

In conclusion, we demonstrated that the introduction of HT functional bioassays in venomic studies is essential to provide a more complete understanding of venom components in terms of structure and function. It also allows venom peptides to be ranked for further investigation based on their bioactivity and structural diversity, which is not possible via transcriptomic and proteomic studies alone. Furthermore, this study provides a guide to assist the exploration of neuroactive venoms from other animals, in particularly for the underexplored araneomorph spiders.

Acknowledgments

We thank Mr. Alun Jones and Dr. Kuok Yap (Institute for Molecular Bioscience, The University of Queensland) for assistance with mass spectrometry experiments.

Funding Statement

This work was supported by The University of Queensland, the Australian National Health and Medical Research Council (Ideas Grant GNT1188959 to FC; Principal Research Fellowship APP1136889 to GK), and the Australian Research Council (Discovery Grant DP200102867 to AW; Centre of Excellence Grant CE200100012 to GK).

Data availability statement

Author contributions.

Conceptualization: FC; design, conduct, and analysis of experiments: FC and AW; MG contributed with the P. nigriventer crude venom. drafting of manuscript: FC. All authors contributed to reviewing and editing of the manuscript and approved the final version for submission.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmolb.2023.1069764/full#supplementary-material

• Almeida A. P., Andrade A. B., Ferreira A. J., Pires A. C., Damasceno D. D., Alves M. N., et al. (2011). Antiarrhythmogenic effects of a neurotoxin from the spider Phoneutria nigriventer . Toxicon 57 , 217–224. 10.1016/j.toxicon.2010.11.013 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Binford G. J., Bodner M. R., Cordes M. H., Baldwin K. L., Rynerson M. R., Burns S. N., et al. (2009). Molecular evolution, functional variation, and proposed nomenclature of the gene family that includes sphingomyelinase D in sicariid spider venoms . Mol. Biol. Evol. 26 , 547–566. 10.1093/molbev/msn274 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Castro J., Grundy L., Schober G., Garcia-Caraballo S., Zhao T., et al. (2021). A spider-venom peptide with multitarget activity on sodium and calcium channels alleviates chronic visceral pain in a model of irritable bowel syndrome . Pain 162 , 569–581. 10.1097/j.pain.0000000000002041 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Dekan Z., Rosengren K. J., Erickson A., Vetter I., Deuis J., et al. (2015). Identification and characterization of ProTx-III [μ-TRTX-Tp1a], a new voltage-gated sodium channel inhibitor from venom of the tarantula Thrixopelma Pruriens . Mol. Pharmacol. 88 , 291–303. 10.1124/mol.115.098178 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Dekan Z., Smith J. J., Deuis J. R., Vetter I., Herzig V., et al. (2017). Modulatory features of the novel spider toxin μ-TRTX-Df1a isolated from the venom of the spider Davus fasciatus . Br. J. Pharmacol. 174 , 2528–2544. 10.1111/bph.13865 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Lewis R. J. (2018). Sodium channels and pain: From toxins to therapies . Br. J. Pharmacol. 175 , 2138–2157. 10.1111/bph.13962 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Lewis R. J. (2019). Structure-function and therapeutic potential of spider venom-derived cysteine knot peptides targeting sodium channels . Front. Pharmacol. 10 , 366. 10.3389/fphar.2019.00366 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C. (2020). Multi-targeting sodium and calcium channels using venom peptides for the treatment of complex ion channels-related diseases . Biochem. Pharmacol. 181 , 114107. 10.1016/j.bcp.2020.114107 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Pacifico L. G., Carvalho D. C., Victoria J. M., Neves A. L., Chavez-Olortegui C., et al. (2003). Molecular cloning and characterization of Phoneutria nigriventer toxins active on calcium channels . Toxicon 41 , 755–763. 10.1016/s0041-0101(03)00011-4 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cardoso F. C., Pineda S. S., Herzig V., Sunagar K., Shaikh N. Y., Jin A., et al. (2022). The deadly toxin arsenal of the tree-dwelling Australian funnel-web spiders . Int. J. Mol. Biosci. 23 ( 21 ), 13077. [ PMC free article ] [ PubMed ] [ Google Scholar ]
• Cavalli J., De Assis P. M., Cristina Dalazen Goncalves E., Daniele Bobermin L., Quincozes-Santos A., Raposo N. R. B., et al. (2022). Systemic, intrathecal, and intracerebroventricular antihyperalgesic effects of the calcium channel blocker CTK 01512-2 toxin in persistent pain models . Mol. Neurobiol. 59 , 4436–4452. 10.1007/s12035-022-02864-w [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Cordeiro Mdo N., De Figueiredo S. G., Valentim Ado C., Diniz C. R., Von Eickstedt V. R., Gilroy J., et al. (1993). Purification and amino acid sequences of six Tx3 type neurotoxins from the venom of the Brazilian 'armed' spider Phoneutria nigriventer (Keys) . Toxicon 31 , 35–42. 10.1016/0041-0101(93)90354-l [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Da Silva C. N., Dourado L. F. N., De Lima M. E., Da Silva Cunha- A., Jr (2020). PnPP-19 peptide as a novel drug candidate for topical glaucoma therapy through nitric oxide release . Transl. Vis. Sci. Technol. 9 , 33. 10.1167/tvst.9.8.33 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• De Lima M. E., Stankiewicz M., Hamon A., De Figueiredo S. G., Cordeiro M. N., Diniz C. R., et al. (2002). The toxin Tx4(6-1) from the spider Phoneutria nigriventer slows down Na + current inactivation in insect CNS via binding to receptor site 3 . J. Insect Physiol. 48 , 53–61. 10.1016/s0022-1910(01)00143-3 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Diniz M. R., Theakston R. D., Crampton J. M., Nascimento Cordeiro M., Pimenta A. M., De Lima M. E., et al. (2006). Functional expression and purification of recombinant Tx1, a sodium channel blocker neurotoxin from the venom of the Brazilian “armed” spider, Phoneutria nigriventer . Protein Expr. Purif. 50 , 18–24. 10.1016/j.pep.2006.06.012 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Diniz M. R. V., Paiva A. L. B., Guerra-Duarte C., Nishiyama M. Y., Jr., Mudadu M. A., Oliveira U., et al. (2018). An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches . PLoS One 13 , e0200628. 10.1371/journal.pone.0200628 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Dos Santos R. G., Van Renterghem C., Martin-Moutot N., Mansuelle P., Cordeiro M. N., Diniz C. R., et al. (2002). Phoneutria nigriventer ω-phonetoxin IIA blocks the Ca V 2 family of calcium channels and interacts with ω-conotoxin-binding sites . J. Biol. Chem. 277 , 13856–13862. 10.1074/jbc.M112348200 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Emerich B. L., Ferreira R. C., Cordeiro M. N., Borges M. H., Pimenta A. M., Figueiredo S. G., et al. (2016). δ-Ctenitoxin-Pn1a, a peptide from Phoneutria nigriventer spider venom, shows antinociceptive effect involving opioid and cannabinoid systems, in Rats . Toxins (Basel) 8 , 106. 10.3390/toxins8040106 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Estrada-Gomez S., Cardoso F. C., Vargas-Munoz L. J., Quintana-Castillo J. C., Arenas Gomez C. M., Pineda S. S., et al. (2019). Venomic, transcriptomic, and bioactivity analyses of Pamphobeteus verdolaga venom reveal complex disulfide-rich peptides that modulate calcium channels . Toxins (Basel) 11 , 496. 10.3390/toxins11090496 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Garcia Mendes M. P., Carvalho Dos Santos D., Rezende M. J. S., Assis Ferreira L. C., Rigo F. K., Jose De Castro Junior C., et al. (2021). Effects of intravenous administration of recombinant Phα1β toxin in a mouse model of fibromyalgia . Toxicon 195 , 104–110. 10.1016/j.toxicon.2021.03.012 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Gewehr C., Oliveira S. M., Rossato M. F., Trevisan G., Dalmolin G. D., Rigo F. K., et al. (2013). Mechanisms involved in the nociception triggered by the venom of the armed spider Phoneutria nigriventer . PLoS Negl. Trop. Dis. 7 , e2198. 10.1371/journal.pntd.0002198 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Hu H., Mawlawi S. E., Zhao T., Deuis J. R., Jami S., Vetter I., et al. (2021). Engineering of a spider peptide via conserved structure-function traits optimizes sodium channel inhibition in vitro and anti-nociception in vivo . Front. Mol. Biosci. 8 , 742457. 10.3389/fmolb.2021.742457 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Isbister G. K., Fan H. W. (2011). Spider bite . Lancet 378 , 2039–2047. 10.1016/S0140-6736(10)62230-1 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Joviano-Santos J. V., Valadao P. a. C., Magalhaes-Gomes M. P. S., Fernandes L. F., Diniz D. M., Machado T. C. G., et al. (2022). Neuroprotective effect of CTK 01512-2 recombinant toxin at the spinal cord in a model of Huntington's disease . Exp. Physiol. 107 , 933–945. 10.1113/EP090327 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Jumper J., Evans R., Pritzel A., Green T., Figurnov M., Ronneberger O., et al. (2021). Highly accurate protein structure prediction with AlphaFold . Nature 596 , 583–589. 10.1038/s41586-021-03819-2 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• King G. F., Gentz M. C., Escoubas P., Nicholson G. M. (2008). A rational nomenclature for naming peptide toxins from spiders and other venomous animals . Toxicon 52 , 264–276. 10.1016/j.toxicon.2008.05.020 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• King G. F., Hardy M. C. (2013). Spider-venom peptides: Structure, pharmacology, and potential for control of insect pests . Annu. Rev. Entomol. 58 , 475–496. 10.1146/annurev-ento-120811-153650 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• King G. F. (2004). The wonderful world of spiders: Preface to the special toxicon issue on spider venoms . Toxicon 43 , 471–475. 10.1016/j.toxicon.2004.02.001 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• King G. F. (2011). Venoms as a platform for human drugs: Translating toxins into therapeutics . Expert Opin. Biol. Ther. 11 , 1469–1484. 10.1517/14712598.2011.621940 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Kono N., Nakamura H., Ohtoshi R., Moran D. a. P., Shinohara A., Yoshida Y., et al. (2019). Orb-weaving spider Araneus ventricosus genome elucidates the spidroin gene catalogue . Sci. Rep. 9 , 8380. 10.1038/s41598-019-44775-2 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Kushmerick C., Kalapothakis E., Beirao P. S., Penaforte C. L., Prado V. F., Cruz J. S., et al. (1999). Phoneutria nigriventer toxin Tx3-1 blocks A-type K + currents controlling Ca 2+ oscillation frequency in GH3 cells . J. Neurochem. 72 , 1472–1481. 10.1046/j.1471-4159.1999.721472.x [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Martin-Moutot N., Mansuelle P., Alcaraz G., Dos Santos R. G., Cordeiro M. N., De Lima M. E., et al. (2006). Phoneutria nigriventer toxin 1: A novel, state-dependent inhibitor of neuronal sodium channels that interacts with micro conotoxin binding sites . Mol. Pharmacol. 69 , 1931–1937. 10.1124/mol.105.021147 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Nunes Da Silva C., Nunes K. P., De Marco Almeida F., Silva Costa F. L., Borges P. V., Lacativa P., et al. (2019). PnPP-19 peptide restores erectile function in hypertensive and diabetic animals through intravenous and topical administration . J. Sex. Med. 16 , 365–374. 10.1016/j.jsxm.2019.01.004 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Paiva A. L., Matavel A., Peigneur S., Cordeiro M. N., Tytgat J., Diniz M. R., et al. (2016). Differential effects of the recombinant toxin PnTx4(5-5) from the spider Phoneutria nigriventer on mammalian and insect sodium channels . Biochimie 121 , 326–335. 10.1016/j.biochi.2015.12.019 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Pedron C., Antunes F. T. T., Rebelo I. N., Campos M. M., Correa A. P., Klein C. P., et al. (2021). Phoneutria nigriventer Tx3-3 peptide toxin reduces fibromyalgia symptoms in mice . Neuropeptides 85 , 102094. 10.1016/j.npep.2020.102094 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Peigneur S., De Lima M. E., Tytgat J. (2018). Phoneutria nigriventer venom: A pharmacological treasure . Toxicon 151 , 96–110. 10.1016/j.toxicon.2018.07.008 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Pipkorn R., Boenke C., Gehrke M., Hoffmann R. (2002). High-throughput peptide synthesis and peptide purification strategy at the low micromol-scale using the 96-well format . J. Pept. Res. 59 , 105–114. 10.1034/j.1399-3011.2002.01958.x [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Pymol (2023). The PyMOL molecular graphics system, version 2.0 schrödinger, LLC . [ Google Scholar ]
• Raposo C., Bjorklund U., Kalapothakis E., Biber B., Alice Da Cruz-Hofling M., Hansson E. (2016). Neuropharmacological effects of Phoneutria nigriventer venom on astrocytes . Neurochem. Int. 96 , 13–23. 10.1016/j.neuint.2016.04.005 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Richardson M., Pimenta A. M. C., Bemquerer M. P., Santoro M. M., Beirao P. S. L., Lima M. E., et al. (2006). Comparison of the partial proteomes of the venoms of Brazilian spiders of the genus Phoneutria . Comp. Biochem. Physiol. C Toxicol. Pharmacol. 142 , 173–187. 10.1016/j.cbpc.2005.09.010 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Smith J. J., Herzig V., King G. F., Alewood P. F. (2013). The insecticidal potential of venom peptides . Cell Mol. Life Sci. 70 , 3665–3693. 10.1007/s00018-013-1315-3 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Smith J. J., Lau C. H. Y., Herzig V., Ikonomopoulou M. P., Rash L. D., King G. F. (2015). “ Therapeutic applications of spider-venom peptides ,” in Venoms to drugs: Venom as a source for the development of human therapeutics (London, United Kingdom: The Royal Society of Chemistry; ), 221–244. [ Google Scholar ]
• Turchetto J., Sequeira A. F., Ramond L., Peysson F., Bras J. L., Saez N. J., et al. (2017). High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery . Microb. Cell Fact. 16 , 6. 10.1186/s12934-016-0617-1 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Vieira L. B., Kushmerick C., Hildebrand M. E., Garcia E., Stea A., Cordeiro M. N., et al. (2005). Inhibition of high voltage-activated calcium channels by spider toxin PnTx3-6 . J. Pharmacol. Exp. Ther. 314 , 1370–1377. 10.1124/jpet.105.087023 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Von Reumont B. M., Anderluh G., Antunes A., Ayvazyan N., Beis D., Caliskan F., et al. (2022). Modern venomics-Current insights, novel methods, and future perspectives in biological and applied animal venom research , 11 . Gigascience [ PMC free article ] [ PubMed ] [ Google Scholar ]
• Wisedchaisri G., Tonggu L., Gamal El-Din T. M., Mccord E., Zheng N., Catterall W. A. (2021). Structural basis for high-affinity trapping of the Na V 1.7 channel in its resting state by tarantula toxin . Mol. Cell 81 , 38–48.e4. 10.1016/j.molcel.2020.10.039 [ PMC free article ] [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Yonamine C. M., Troncone L. R., Camillo M. A. (2004). Blockade of neuronal nitric oxide synthase abolishes the toxic effects of Tx2-5, a lethal Phoneutria nigriventer spider toxin . Toxicon 44 , 169–172. 10.1016/j.toxicon.2004.05.016 [ PubMed ] [ CrossRef ] [ Google Scholar ]
• Zhang Y., Chen J., Tang X., Wang F., Jiang L., Xiong X., et al. (2010). Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis . Zool. (Jena) 113 , 10–18. 10.1016/j.zool.2009.04.001 [ PubMed ] [ CrossRef ] [ Google Scholar ]